These Lbp knock-out mice exhibit unresponsiveness to intraperitoneal injections of lipopolysaccharide.
Robert S. Jack, Klinikum der Uni Greifswald
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) activity is detected by ELISA analysis of serum. Homozygous mice are unresponsive to intraperitoneal injections of lipopolysaccharide (LPS)(200ng) even when pre-treated with galactosamine. Serum concentrations of TNF-alpha remain low after LPS challenge. Gram-negative bacteria infection induced by intraperitoneal injections of Salmonella typhimurium is fatal to mutant mice due to an impaired inflammatory response. This mutant mouse strain may be useful in studies of immune responses to bacterial infection.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the exon encoding alanine 43 to serine 55 of the targeted gene. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts.
|Allele Name||targeted mutation 1, Robert S Jack|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LBP -; Lbptm1Csc|
|Gene Symbol and Name||Lbp, lipopolysaccharide binding protein|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neomycin resistance gene was inserted into an exon encoding alanine 43 to serine 55. No LBP activity was detectable by ELISA assay in serum derived from homozygous mice.|
|Mutations Made By|| |
Robert Jack, Klinikum der Uni Greifswald
The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to the BALB/c background for 10 generations before being made homozygous. While maintaining a live colony, these mice were bred as homozygotes.
When using the LBP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004647 in your Materials and Methods section.