DBA/2J-Grxcr1^pi-2J/J

Stock number: 004641
Product name: pirouette 2 Jackson
Research areas: Neurobiology Research, Sensorineural Research

Detailed description

Mice homozygous for the pirouette 2 Jackson mutation display bi-directional circling and head bobbing and are completely deaf by ABR assessment. Consistent with a vestibular defect, they fail to swim at the surface of water, but instead roll underwater and sink before rescue.

Development

The pirouette 2 Jackson mutation arose spontaneously in the DBA/2J inbred colony at The Jackson Laboratory in 1999 and was maintained by sibling inbreeding on this background. In 2003 sperm were cryopreserved from homozygous males at generation F10.

Allele

Symbol: Grxcr1^pi-2J
Name: pirouette 2 Jackson
MGI accession ID: MGI:3027304
Generation method: Spontaneous
Marker symbol: Grxcr1
Marker name: glutaredoxin, cysteine rich 1
Marker synonyms: DFNB25; pi; PPP1R88; RGD1564635; tde
Chromosome: 5
Strain of origin: DBA/2J
Molecular note: Genomic PCR demonstrated a deletion with a maximum size of approximately 200 kb that includes exon 1, nearly 125 kb of upstream sequence, and the majority of intron 1. The deletion breakpoint in intron 1 is within 12 kb of exon 2.