Mice homozygous for this recessive mutation are recognized by their wasting pre-weaning lethal phenotype, they curl up, waste away and die by 21 days. Mutants do not live to breed but no lesions have been found pathologically.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Vamp1 | vesicle-associated membrane protein 1 |
Mice homozygous for this recessive mutation are recognized by their wasting pre-weaning lethal phenotype, they curl up, waste away and die by 21 days. VAMP1 mRNA was found to be significantly downregulated in the lethal-wasting brain compared to wild-type littermates. Mutants do not live to breed but no lesions have been found pathologically.
The lew mutation arose spontaneously in 1985 in a mutation-bearing C3H/HeDiSnJ subline at The Jackson Laboratory and has been maintained on the same background.
Allele Name | lethal wasting |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | Syb1lew |
Gene Symbol and Name | Vamp1, vesicle-associated membrane protein 1 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeSnJ |
Chromosome | 6 |
Molecular Note | A spontaneous mutant identified at The Jackson Laboratory. A c.190G>T transversion creates a premature stop codon at glutamic acid codon 64 (p.E64*). The predicted protein that results would lack both a transmembrane domain and a calmodulin interaction domain. mRNA levels are severely reduced and no protein can be detected by immunohistochemistry in the brain. |
Because mice homozygous for this recessive mutation die by wean age, this strain needs to be progeny tested at each generation to identify heterozygous breeder pairs.
When using the C3H/HeSnJ-Vamp1lew/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #004626 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Vamp1<lew> |
Frozen Mouse Embryo | C3H/HeSnJ-Vamp1<lew>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C3H/HeSnJ-Vamp1<lew>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C3H/HeSnJ-Vamp1<lew>/GrsrJ | $3373.50 |
Frozen Mouse Embryo | C3H/HeSnJ-Vamp1<lew>/GrsrJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.