These TOPGAL transgenic mice express Beta-galactosidase as a reporter in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 mediated signaling pathway and activated Beta-catenin. Beta-galactosidase activity is displayed during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles, This strain represents an effective tool for generating mutants that would be useful in studies of the Wnt signaling pathway.
Elaine Fuchs, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Gnat2 | guanine nucleotide binding protein, alpha transducing 2 |
This strain may be homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.
These TOPGAL transgenic mice are a reporter strain that express Beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF) mediated signaling pathway and activated Beta-catenin. The transgene contains the lacZ gene under the control of a regulatory sequence consisting of three consensus LEF/TCF-binding motifs upstream of a minimal c-fos promoter. Transgenic mice display TOPGAL activity (Beta-galactosidase activity) during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles, but not during the next stage of hair follicle development; formation of hair germs. TOPGAL transgene activity reappears in hair follicles at E16.5 and TOPGAL expression is strongly upregulated in the postnatal hair shaft precursor cells in both whisker and body hair anagen follicles (active periods of hair growth). TOPGAL expression ceases during catagen (regression and shortening) and telogen (rest) periods of the postnatal hair growth cycle. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating mutants that would be useful in studies of the Wnt signaling pathway.
The TOPGAL transgene was designed by Dr. Elaine Fuchs (while at The University of Chicago) with three copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) consensus binding site and the FBJ osteosarcoma oncogene (Fos; c-fos) minimal promoter upstream of the β-galactosidase gene (lacZ). In 2003, TOPGAL mice on a CD-1 genetic background were sent to The Jackson Laboratory Repository as Stock No. 004623. This strain may be homozygous for Gnat2cpfl3 (cone photoreceptor function loss 3), which affects bright light (photopic) vision.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression occurs during early embryonic development in a subset of pluripotent embryonic basel cells of the epithelium and dermis of developing hair follicles. lacZ expression disappears during formation of hair germ and then reappears at E16.5 in hair follicles until 18 days after birth. |
Site of Expression |
Allele Name | transgene insertion 34, Elaine Fuchs |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | TCF-betagal; Tg(Fos-lacZ)34Efu; TOPGAL; Top-Gal |
Gene Symbol and Name | Tg(TCF/Lef1-lacZ)34Efu, transgene insertion 34, Elaine Fuchs |
Gene Synonym(s) | |
Promoter | Fos, Fos proto-oncogene, AP-1 transcription factor subunit, rat |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression occurs during early embryonic development in a subset of pluripotent embryonic basel cells of the epithelium and dermis of developing hair follicles. lacZ expression disappears during formation of hair germ and then reappears at E16.5 in hair follicles until 18 days after birth. |
Strain of Origin | Not Specified |
Chromosome | UN |
General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgenic mice express beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF1/TCF3) mediated signaling pathway and activated Beta-catenin (CATNB). Transgenic mice display Beta-galactosidase activity during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles. Beta-galactosidase activity is not detected in the next stage of hair follicle development, formation of hair germs. At E16.5, transgene activity reappears in hair follicles and is detectable until 18 days after birth. |
Molecular Note | The transgene contains the lacZ gene under the control of a promoter consisting of three consensus lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF)-binding motifs upstream of a minimal Fos promoter. This allele is responsive to canonical Wnt/beta-catenin signal transduction. |
Mutations Made By | Elaine Fuchs, The Rockefeller University |
Allele Name | cone photoreceptor function loss 3 |
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Allele Type | Spontaneous |
Allele Synonym(s) | no cones |
Gene Symbol and Name | Gnat2, guanine nucleotide binding protein, alpha transducing 2 |
Gene Synonym(s) | |
Strain of Origin | various |
Chromosome | 3 |
General Note | This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428) The allele has also been reported in NMRI and CD1 (Lluis Montoliu, J:212307) |
Molecular Note | A single nucleotide substitution of G to A at coding nucleotide 598 in exon 6. This mutation converts codon 200 from aspartic acid to asparagine (p.D200N). |
When maintaining a live colony, hemizygous mice may be bred together or to wildtype (noncarrier) mice from the colony. The coat color expected from breeding is Albino.
When using the TOPGAL mouse strain in a publication, please cite the originating article(s) and include JAX stock #004623 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fos-lacZ)34Efu, , 1 pair minimum |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-lacZ)34Efu/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-lacZ)34Efu/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-lacZ)34Efu/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-lacZ)34Efu/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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