Homozygotes for this transgene are not viable. Hemizygotes express Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target, and recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.
Dr. Albee Messing, University of Wisconsin-Madison
Genetic Background | Generation |
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N?+1pN33F2
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.
View Cre expression characterization.
Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that both female and male GFAP-Cre;floxed double mutants bred to floxed mice produced some offspring with germline deletion of the floxed allele [42.9% (6/14) and 50% (9/18) when using Cre female or Cre male, respectively, including some Cre negative offspring]. As such, for Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider breeding GFAP-Cre hemizygous mice to floxed mice.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. For endogenous mouse gene expression, information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.
A transgenic construct containing a 2.2Kb 5' flanking region of the human GFAP gene, cre coding sequence, the SV40 nuclear localization signal, and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site, was injected into fertilized FVB/N mouse eggs.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver |
Allele Name | transgene insertion 25, Albee Messing |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Gfa2-Cre; GFAP-Cre; GFAP-Cre-m; hGFAP::Cre; hGFAPcre; hGFAP-cre; Tg95.2; TgN(GFAPcre)25Mes; TgN25Mes |
Gene Symbol and Name | Tg(GFAP-cre)25Mes, transgene insertion 25, Albee Messing |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. |
Molecular Note | This transgene expresses Cre recombinase under the control of a human glial fibrillary acidic protein (GFAP) promoter. Cre-mediated recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. |
Mutations Made By | Dr. Albee Messing, University of Wisconsin-Madison |
The strain is maintained as a hemizygote on the FVB/N background.
For Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider breeding GFAP-Cre hemizygous mice to floxed mice. See Detailed Description for more details.
When using the FVB-Tg(GFAP-cre)25Mes/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004600 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(GFAP-cre)25Mes |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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