Overview

Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.

Homozygotes for this transgene are not viable. Hemizygotes express Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target, and recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.

Donating Investigator

Dr. Albee Messing, University of Wisconsin-Madison

Genetic overview

Genetic Background	Generation
	N?+1pN33F2 (2021-01-02 00:00:00)

Tg(GFAP-cre)25Mes

Allele Type
Transgenic (Recombinase-expressing)

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female 4-week

356.51 Domestic price for breeder pair

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.

View Cre expression characterization.

Luo et al. 2020 Neuron 106:37 Table 1 shows germline recombination in offspring (F2) of Cre;floxed double mutant (F1) mice bred to floxed and/or wildtype mice. The authors also note that in general, the frequency of recombination in Cre;floxed double mutant germline cells appears to be considerably higher than in zygotes produced by breeding Cre mice to floxed mice.
This reports that both female and male GFAP-Cre;floxed double mutants bred to floxed mice produced some offspring with germline deletion of the floxed allele [42.9% (6/14) and 50% (9/18) when using Cre female or Cre male, respectively, including some Cre negative offspring]. As such, for Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider breeding GFAP-Cre hemizygous mice to floxed mice.

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry. For endogenous mouse gene expression, information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.

Development

A transgenic construct containing a 2.2Kb 5' flanking region of the human GFAP gene, cre coding sequence, the SV40 nuclear localization signal, and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site, was injected into fertilized FVB/N mouse eggs.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver

Control Suggestions

Noncarrier
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Zhuo L; Theis M; Alvarez-Maya I; Brenner M; Willecke K; Messing A. 2001. hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo. Genesis 31(2):85-94PubMed: 11668683 MGI: J:72509

View All References

Genetics

Tg(GFAP-cre)25Mes

Allele Symbol: Tg(GFAP-cre)25Mes

Allele Name	transgene insertion 25, Albee Messing
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	Gfa2-Cre; GFAP-Cre; GFAP-Cre-m; hGFAP::Cre; hGFAPcre; hGFAP-cre; Tg95.2; TgN(GFAPcre)25Mes; TgN25Mes
Gene Symbol and Name	Tg(GFAP-cre)25Mes, transgene insertion 25, Albee Messing
Gene Synonym(s)
Promoter	GFAP, glial fibrillary acidic protein, human
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver
Strain of Origin	FVB/N
Chromosome	UN
General Note	Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable.
Molecular Note	This transgene expresses Cre recombinase under the control of a human glial fibrillary acidic protein (GFAP) promoter. Cre-mediated recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase.
Mutations Made By	Dr. Albee Messing, University of Wisconsin-Madison

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
- Neurobiology Research
- Cre-lox System
  - Cre Recombinase Expression
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Zhuo L; Theis M; Alvarez-Maya I; Brenner M; Willecke K; Messing A. 2001. hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo. Genesis 31(2):85-94PubMed: 11668683 MGI: J:72509

Additional References

Moore SA; Saito F; Chen J; Michele DE; Henry MD; Messing A; Cohn RD; Ross-Barta SE; Westra S; Williamson RA; Hoshi T; Campbell KP. 2002. Deletion of brain dystroglycan recapitulates aspects of congenital muscular dystrophy. Nature 418(6896):422-5PubMed: 12140559 MGI: J:86901

Additional - Tg(GFAP-cre)25Mes related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Standard PCR:Tg(GFAP-cre)25Mes
- Standard PCR:Tg(GFAP-cre)25Mes
- Standard PCR:Tg(GFAP-cre)25Mes
- Standard PCR:Generic Cre Melt Curve Analysis
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

The strain is maintained as a hemizygote on the FVB/N background.

For Cre-lox experiments and to reduce the frequency of germline deletion of the floxed allele, researchers may consider breeding GFAP-Cre hemizygous mice to floxed mice. See Detailed Description for more details.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
- Hemizygote x Noncarrier
Citation
When using the FVB-Tg(GFAP-cre)25Mes/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004600 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or Non carrier for Tg(GFAP-cre)25Mes

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(GFAP-cre)25Mes

Allele Symbol: Tg(GFAP-cre)25Mes

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Tg(GFAP-cre)25Mes related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information