| Allele Name | transgene insertion 14-2, Helen H Hobbs |
| Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | ABCG5/ABCG8; G5G8Tg; hG5G8Tg |
| Gene Symbol and Name | Tg(ABCG5/ABCG8)14-2Hobb, transgene insertion 14-2, Helen H Hobbs |
| Gene Synonym(s) | |
| Promoter | ABCG8, ATP binding cassette subfamily G member 8, human |
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| Promoter | ABCG5, ATP binding cassette subfamily G member 5, human |
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| Expressed Gene | ABCG8, ATP binding cassette subfamily G member 8, human |
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| Expressed Gene | ABCG5, ATP binding cassette subfamily G member 5, human |
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| Strain of Origin | (C57BL/6J x SJL)F2 |
| Chromosome | UN |
| Molecular Note | The transgene contains a 90kb of sequence including the entire coding regions of the human ABCG5 and ABCG8 genes, approximately 30Kb of sequence flanking the 3' end of ABCG5, 6Kb of sequence flanking the 3' end of ABCG8 and sequence encoding exons 6 to 13 of a putative gene called CGI-60. Estimated transgene copy number is ~14 for this line. Northern blot analysis revealed the human transgene is expressed in the liver and small intestine. RT-PCR showed trace levels of transgene transcript in ovary tissue. |
| Mutations Made By | Liqing Yu, Wake Forest Univ. School of Medicine |
