Smoc mice have loxP sites flanking exon 1 of the smoothened homolog (Drosophila) gene. Exposure to Cre recombinase removes the floxed sequence - creating a null allele. These Smoc mice may be useful in studying hedgehog signaling and cell proliferation in the dental epithelium, craniofacial development, astrocyte populations, cardiovascular development, and of cardiac neural crest cells.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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?+F1pF7
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) | Smo | smoothened, frizzled class receptor |
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the skin (Stock No. 004782), this mutant mouse strain may be useful in studies of hedgehog signalling and cell proliferation in the dental epithelium.
When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the nervous system (Stock No. 003771), this mutant mouse strain may be useful in studies of hedgehog signalling and cerebellar foliation. It has been the experience of The Jackson Laboratory Repository that homozygous mice exhibit small eyes.
When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the midbrain/dorsal spinal cord (Stock No. 007807), this mutant mouse strain may be useful in studies of craniofacial development.
When bred to a strain expressing Cre recombinase in astrocytes and neural stem cells (see Stock No. 012886 for example), this mutant mouse strain may be useful in studies of specific populations of astrocytes.
When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of cardiovascular development.
When bred to a strain expressing Cre recombinase in the neural tube/midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of cardiac neural crest cells.
A loxP site flanked targeting vector containing a 400bp fragment of exon 1 and an FRT-flanked neomycin resistance gene was utilized in the construction of this mutant. This construct, with a downstream tau-lacZ, was electroporated into 129X1/SvJ derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to Swiss Webster females. The strain was then maintained as a homozygote prior to sending to the Jackson Laboratory in 2002.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | When bred with mice containing Cre recombinase, lacZ will be expressed in Cre-expressing cells of the offspring. |
Allele Name | targeted mutation 2, Andrew P McMahon |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, No functional change) |
Allele Synonym(s) | Smoc; Smof; Smofl; Smoflox |
Gene Symbol and Name | Smo, smoothened, frizzled class receptor |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | When bred with mice containing Cre recombinase, lacZ will be expressed in Cre-expressing cells of the offspring. |
Strain of Origin | 129X1/SvJ |
Chromosome | 6 |
Molecular Note | Two loxP sites and an frt-flanked neomycin resistance cassette were inserted flanking ~400 bp of the first coding exon 1 from 44 bp upstream through 358 bp downstream of the translation initiation codon. A tau-LacZ gene was also inserted 3' to the neomycin resistance gene. |
Mutations Made By | Joe Vaughan, Harvard University |
When maintaining a live colony, these mice can be bred as homozygotes.
[2020]
The original coat color expected from breeding was Agouti (colony may be still segregating for albino). [2002]
When using the Smoc mouse strain in a publication, please cite the originating article(s) and include JAX stock #004526 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for Smo<tm2Amc>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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