These Bcl2l11tm1.1Ast knock-out mice exhibit progressive systemic autoimmune disease. They may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.
Andreas Strasser, Walter and Eliza Hall Inst of Med Res
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Bcl2l11 | BCL2-like 11 (apoptosis facilitator) |
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No full length gene product (protein) is immunodetected in spleen cells from homozygous mutant mice. Homozygous mice have lympho-myeloid hyperplasia and reduced platelet number. Lymphocytes are insensitive to certain apoptotic stimuli. Both homozygous and heterozygous mice exhibit progressive systemic autoimmune disease. This mutant mouse strain may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.
A targeting vector containing a floxed neomycin resistance gene and a thymidine kinase gene was used to disrupt the exon encoding the BH3 domain. The construct was electroporated into 129S1 derived W9.5 embryonic stem (ES) cells. correctly targeted ES cells were injected into C57BL/6 blastocysts.
Allele Name | targeted mutation 1.1, Andreas Strasser |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | bim- |
Gene Symbol and Name | Bcl2l11, BCL2-like 11 (apoptosis facilitator) |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 2 |
Molecular Note | Insertion of a floxed PGK-neomycin cassette deleted the exon encoding the BH3 domain. Cre-mediated recombination in vivo subsequently removed the inserted cassette. In the final allele a single loxP site remains in place of the exon. Immunoblot analysis using a rat monoclonal antibody did not detect full-length protein, but did detect a small amount of a truncated polypeptide in spleen cells from homozygous mutant mice. |
Mutations Made By | Andreas Strasser, Walter and Eliza Hall Inst of Med Res |
The resulting chimeric animals were crossed to a C57BL/6 Cre-deleter strain to remove the neo cassette, and then backcrossed to C57BL/6 for 13 generations. Donating Investigator reports that homozygous females, although fertile, tend to be poor mothers; uses the following breeding scheme: heterozygous females X homozygous males . 5-7 pups per litter.
When using the Bim KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004525 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bcl2l11<tm1.1Ast> |
Frozen Mouse Embryo | B6.129S1-Bcl2l11<tm1.1Ast>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Bcl2l11<tm1.1Ast>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Bcl2l11<tm1.1Ast>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Bcl2l11<tm1.1Ast>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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