These Bcl2l11tm1.1Ast knock-out mice exhibit progressive systemic autoimmune disease. They may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.
Andreas Strasser, Walter and Eliza Hall Inst of Med Res
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No full length gene product (protein) is immunodetected in spleen cells from homozygous mutant mice. Homozygous mice have lympho-myeloid hyperplasia and reduced platelet number. Lymphocytes are insensitive to certain apoptotic stimuli. Both homozygous and heterozygous mice exhibit progressive systemic autoimmune disease. This mutant mouse strain may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.
A targeting vector containing a floxed neomycin resistance gene and a thymidine kinase gene was used to disrupt the exon encoding the BH3 domain. The construct was electroporated into 129S1 derived W9.5 embryonic stem (ES) cells. correctly targeted ES cells were injected into C57BL/6 blastocysts.
|Allele Name||targeted mutation 1.1, Andreas Strasser|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Bcl2l11, BCL2-like 11 (apoptosis facilitator)|
|Gene Synonym(s)||1500006F24Rik; 1500006F24Rik; BAM; BIM; BOD; Bcl2 interacting mediator of cell death; Bim; Bim; BimL; Bod; RIKEN cDNA 1500006F24 gene|
|Strain of Origin||129S1/Sv-Oca2<+> Tyr<+> Kitl<+>|
|Molecular Note||Insertion of a floxed PGK-neomycin cassette deleted the exon encoding the BH3 domain. Cre-mediated recombination in vivo subsequently removed the inserted cassette. In the final allele a single loxP site remains in place of the exon. Immunoblot analysis using a rat monoclonal antibody did not detect full-length protein, but did detect a small amount of a truncated polypeptide in spleen cells from homozygous mutant mice.|
|Mutations Made By|| |
Andreas Strasser, Walter and Eliza Hall Inst of Med Res
The resulting chimeric animals were crossed to a C57BL/6 Cre-deleter strain to remove the neo cassette, and then backcrossed to C57BL/6 for 13 generations. Donating Investigator reports that homozygous females, although fertile, tend to be poor mothers; uses the following breeding scheme: heterozygous females X homozygous males . 5-7 pups per litter.
|Please inquire about possible genotypes.|
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