Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of bone marrow-derived cell lysates. There is a 65% reduction of splenocyte number. Development of B lymphocytes is arrested at the B220+CD43+ progenitor B cell to B220+CD43- precursor B cell transition stage. The reduced number of mature peripheral B-cells results in diminished serum Ig levels in adult homozygous mice. Peritoneal CD5+IgM+ B-1a B-cell numbers are decreased. This mutant mouse strain may be useful in studies of agammaglobulinemia.
A targeting vector containing a floxed PGK-neo cassette and Green Fluorescent Protein sequence was used to disrupt exon 1, which encodes amino acids 1-60 and the initiation codon. The construct was electroporated into 129X1/SvJ derived BL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
|Allele Name||targeted mutation 1, Andrew C Chan|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Blnk, B cell linker|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exon 1 was disrupted by homologous recombination of a construct containing GFP, a floxed neo gene, and a mutated start codon (ATC). While the GFP coding region was linked to its own Kozak sequence and start codon, fluorescence was undetected in splenocytes and bone marrow cells derived from heterozygous mutant mice. Immunoblot analysis on homozygote bone marrow cells failed to detect Blnk protein.|
|Mutations Made By|| |
Andrew Chan, Genentech
The resulting chimeric animals were crossed to C57BL/6 mice for 10 generations. Specific pathogen-free (SPF) conditions are recommended.
When using the BLNK KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004524 in your Materials and Methods section.