Overview

Also Known As: long bone abnormality

These mice carry a spontaneous mutation at the Nppc locus characterized by proportionate dwarfing of organs and shorter tails and long bones.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Nppc^lbab

Allele Type	Gene Symbol	Gene Name
Spontaneous	Nppc	natriuretic peptide type C

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Research Applications

Research Tools
Developmental Biology Research
Reproductive Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

The C57BL/6J background strain is homozygous for Cdh23^ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset after 10 months of age. The retinal degeneration 1 mutation, Pde6b^rd1, of the PL/J donor strain may still be segregating in this incipient congenic.

Development

The lbab mutation arose spontaneously on the PL/J inbred strain at The Jackson Laboratory in 1996 when that inbred was at generation F171. Because this mutation was difficult to maintain on the PL/J background, it was immediately backcrossed onto C57BL/6J. For the most part, this has been done by breeding heterozygotes with C57BL/6J mates then progeny testing the offspring to identify heterozygotes to continue the backcross. This strain reached F171+N6F1 in 2004.

Control Suggestions

Untyped from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Ward-Bailey PF; Harris BS; Donahue LR; Bronson R; Johnson KR. 2002. Long bone abnormality (lbab): a new spontaneous mutation causing small size and skeletal abnormalities on Chromosome 1 in the mouse. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine MGI Direct Data SubmissionMGI: J:79175

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Genetics

Nppc^lbab

Allele Symbol: Nppc^lbab

Allele Name	long bone abnormality
Allele Type	Spontaneous
Allele Synonym(s)	long bone abnormality; Nppc^lbab
Gene Symbol and Name	Nppc, natriuretic peptide type C
Gene Synonym(s)	natriuretic peptide precursor C; CNP; CNP2; lbab; lbab; long bone abnormality
Strain of Origin	PL/J
Chromosome	1
Molecular Note	lbab is a spontaneous mutation identified at The Jackson Laboratory. A C-to-G transversion mutation in exon 2 is predicted to result in the substitution of arginine (R) for glycine (G) in a conserved domain of the encoded protein.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Nppc^lbab related

Research Tools
- Reproductive Biology Research
  - oocyte
Developmental Biology Research
- Growth Defects
  - Growth Defects (homozygous)
- Skeletal Defects
Reproductive Biology Research
- Developmental Defects Affecting Gonads
  - females only

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Nppc^lbab/Nppc^lbab
either: (involves: C57BL/6J * PL/J) or PL/J

endocrine/exocrine gland phenotype

small testis
- one month old homozygous males have exceptionally small testes

growth/size/body region phenotype

decreased body size
- overall smaller body size than their littermates; proportionate dwarfing of all organs with the possible exception of the male reproductive tract

disproportionate dwarf
- two mutants examined at 9 weeks of age exhibited a greater long bone: spine proportionality ratio than younger mice, suggesting that the long bones had ceased growing (progressive dwarfism)

limbs/digits/tail phenotype

short fibula
- long bones are shorter than normal

short tail
- mutants have proportionately shortened tails

short tibia
- long bones are shorter than normal

mammalian phenotype

reproductive system phenotype
- Normal - mature mutant males breed, and testes appear by palpation to be of normal size; testis biopsy of a homozygous male revealed normal sperm heads and many sperm tails

reproductive system phenotype

small testis
- one month old homozygous males have exceptionally small testes

skeleton phenotype

abnormal long bone epiphyseal plate morphology
- plates; the growth plates are less affected on C57BL/6J than on the original PL/J background
- about 50% of those examined had growth plate abnormalities, while the rest exhibited normal growth plates
- Background Sensitivity - the growth plates are less affected on C57BL/6J than on the original PL/J background

decreased length of long bones

disorganized long bone epiphyseal plate
- of those having abnormalities, some showed disorganization of both femoral and tibial growth plates while others of only the femoral growth plates;

short fibula
- long bones are shorter than normal

short tibia
- long bones are shorter than normal

Genotype: Nppc^lbab/Nppc^lbab
involves: PL/J

nervous system phenotype

abnormal sensory neuron innervation pattern
- unlike in wild-type mice, axons entering the spinal cord and turn either in rostral or caudal directions without leaving the funiculus

References

Ward-Bailey PF; Harris BS; Donahue LR; Bronson R; Johnson KR. 2002. Long bone abnormality (lbab): a new spontaneous mutation causing small size and skeletal abnormalities on Chromosome 1 in the mouse. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine MGI Direct Data SubmissionMGI: J:79175

Additional - Nppc^lbab related

Technical Support

Contact Technical Support

Genotyping Protocols
- Pyrosequencing:Nppc^lbab
- Genotyping resources and troubleshooting
Breeding Considerations

Homozygotes have not been found to breed.
- Additional Breeding and Husbandry Support
Appearance
- black, small
  Related Genotype: a/a lbab/lbab
  
  black, unaffected
  Related Genotype: a/a lbab/+ or a/a +/?
Citation
When using the long bone abnormality mouse strain in a publication, please cite the originating article(s) and include JAX stock #004521 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Nppc<lbab>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: long bone abnormality

Genetic overview

Research Applications

Base Price

Important Note

Development

Control Suggestions

Additional Information

Selected References

Nppclbab

Allele Symbol: Nppclbab

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Nppclbab related

Mammalian Phenotype Terms by Genotype

Genotype: Nppclbab/Nppclbabeither: (involves: C57BL/6J * PL/J) or PL/J

endocrine/exocrine gland phenotype

growth/size/body region phenotype

limbs/digits/tail phenotype

mammalian phenotype

reproductive system phenotype

skeleton phenotype

Genotype: Nppclbab/Nppclbabinvolves: PL/J

nervous system phenotype

References

Additional - Nppclbab related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Nppc^lbab

Allele Symbol: Nppc^lbab

Genotype: Nppc^lbab related

Genotype: Nppc^lbab/Nppc^lbab
either: (involves: C57BL/6J * PL/J) or PL/J

Genotype: Nppc^lbab/Nppc^lbab
involves: PL/J

Additional - Nppc^lbab related