Male Tnp1 knock-out mice exhibit abnormal sperm development.
Dr. Marvin L. Meistrich, University of Texas M.D. Anderson Cancer
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnp1 | transition protein 1 |
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected in testicular homogenates. Levels of transition nuclear protein 2 and protamine 2 precursor are elevated. Homozygous female mice are fertile. Homozygous male mice are subfertile, approximately 60% are infertile, and display diminished sperm motility, sperm tail abnormalities and sperm chromatin abnormalities. Electron microscopy analysis of spermatids reveals both abnormal large rod-like chromatin condensation units as well as normal fine fibrillar chromatin morphology. This mutant mouse strain may be useful in studies of male infertility and/or subfertility.
A targeting vector containing neomycin resistance and thymidine kinase genes was used to replace the entire targeted gene. The construct was electroporated into 129S7/SvEvBrd-Hprt+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were backcrossed to 129S6/SvEvTac female mice.
Allele Name | targeted mutation 1, Marvin L Meistrich |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Tnp1-; Tnp1-null |
Gene Symbol and Name | Tnp1, transition protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 1 |
Molecular Note | The entire gene was replaced with a neomycin cassette, which was inserted in an antisense orientation. Tnp1 protein was not detectable in extracts of testicular homogenates from homozygous mutant mice. |
Mutations Made By | Dr. Marvin Meistrich, University of Texas M.D. Anderson Cancer |
This strain originated on a 129S7 background, has been backcrossed to the 129S6/SvEvTac background and is maintained as heterozygous males and homozygous females.
When using the Tnp1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004511 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Homozygous or Wild-type for Tnp1<tm1Mlm> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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