These mice harbor a C57BL/6JOrl chromosome segment containing the Klrb1ca allele that determines the Nk1.1 antigen on natural killer cells. Diabetes incidence is significantly decreased in these mice.
Claude Carnaud, INSERM
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | Klrb1c | killer cell lectin-like receptor subfamily B member 1C |
Marker Symbol | Marker Name | |
---|---|---|
D6Mit254 | DNA segment, Chr 6, Massachusetts Institute of Technology 254 | |
D6Mit339 | DNA Segment, Chr 6, Massachusetts Institute of Technology 339 |
Klrb1ca homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Diabetes incidence as reported by donating investigator and confirmed in the T1DR, prior to shortening the congenic interval, is significantly decreased (30% females and 0% males at 30 weeks of age) as compared to standard NOD (NK1.2) controls (85% females and 60% males at 30 weeks of age) . FACS analysis of NKT cells derived from thymus or spleen of NOD animals homozygous for this genomic segment revealed the presence of NK1.1+/TCR alpha beta +, V beta8+ and CD44+, but at lower levels than in C57BL/6 controls. NK cells of NOD.B6-(D6Mit254-D6Mit14) exhibit an equivalent number of NK1.1/DX5 double positive cells when compared to C57BL/6. Histological examination of 10-12 week old animals indicates no significant difference in insulitis severity between NOD.B6-(D6Mit254-D6Mit14) and NOD control islets (Carnaud et al, 2001). Confirming the published report that insulitis development in NK1.1 congenics is not significantly different from control NOD (NK1.2+)(Carnaud et al, 2001), sampling of non-diabetic NOD.NK1.1 congenic mice in the TIDR at 30 wk of age also showed extensive insulitis. This model is useful for looking at the role of natural killer cells in autoimmune diseases such as Type 1 Diabetes.
A Chromosome 6 genomic segment from C57BL/6JOrl originally reported to span D6Mit254 through D6Mit14 and containing Klrb1ca (commonly referred to as NK1.1) was transferred through 10 generations of backcrossing to NOD/Necker (Carnaud et al, 2001). In 2002, this strain arrived at TJL at N10F?. Further a selected genome scan ("Idd sweep" and "Chromosome of interest") indicated that some animals of this strain were heterozygous for a C57BL/6-derived marker on Chromosome 3(D3Mit95) as well as carrying a longer Chromosome 6 congenic interval (D6Mit328 - D6Mit15) than originally published. These animals were backcrossed 4 times to NOD/LtJ (JR1976) prior to making the stock homozygous for a smaller C57BL/6JOrl-derived interval (D6Mit254-D6Mit339) around NK1.1 (62.1 cM) and to eliminate heterozygosity at D3Mit95.
Allele Name | antigen Nk-1.1 allele |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | nk1.1 |
Gene Symbol and Name | Klrb1c, killer cell lectin-like receptor subfamily B member 1C |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 6 |
Molecular Note | The allele Nk1a determines the antigen, designated Nk-1.1, detected by an antibody produced in (BALB/c x C3H)F1 mice against CE lymphoid cells; it occurs in strains CE, C57BL/6, C57BR/cd, C57L, C58, DBA/1, MA/My, NZB, SJL, SM, and B10.D2. |
Mutations Made By | Claude Carnaud, INSERM |
Marker Synonym(s) | |
---|---|
Chromosome(s) | 6 |
Marker Synonym(s) | |
---|---|
Chromosome(s) | 6 |
When using the NOD.B6-(D6Mit254-D6Mit339)/CarJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #004482 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit339)/CarJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit339)/CarJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit339)/CarJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD.B6-(D6Mit254-D6Mit339)/CarJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.