These mice carry an ENU-induced mutation characterized by muscle weakness, abnormal gait, body tremors, and early mortality.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | nmf67 | neuroscience mutagenesis facility, 67 |
The mutants are characterized by muscle weakness and abnormal gait, which become apparent at four weeks of age (average onset 4 +/-0.5. weeks, n=74); an intense body tremor can be observed at 3 weeks or younger. The animals die relatively young (at approximately 6-9 weeks of age), i.e. prior to most phenotype screening, and a colony needs to be maintained through ovarian transplants.
Whole muscle mounts of the hind limb of a mutant (23 days of age) labeled with fluorescent-labeled bungarotoxin, SV2, and SMI 31 revealed abnormal formation of the neuromuscular junctions. The receptors appeared broken up and have not resolved into a smooth continuous shape. Standard pathology work-up on six additional mutants (23-30 days of age) showed vacuolation in the white matter of the medulla, cerebellum, and spinal cord. In addition, the eyes of affected mutants showed multi-focal abnormalities in the
retina.
Nuclei of the outer nuclear layer were displaced into the photoreceptor layer. Histology performed on one dead pup (~24 hours old) revealed a high septal defect in the heart. Hearts from the other mutants appeared normal.
Allele Name | neuroscience mutagenesis facility, 67 |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | |
Gene Symbol and Name | nmf67, neuroscience mutagenesis facility, 67 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | This phenotypic mutant was identified in an ENU mutagenesis screen. |
When using the C57BL/6J-nmf67/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004472 in your Materials and Methods section.
Facility Barrier Level Descriptions
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Unknown for nmf67 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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