B6.129P2-Tnfrsf8^tm1Mak/J

Stock number: 004457
Product name: CD30-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Tnfrsf8 knock-out mice exhibit an increased number of thymocytes.

Detailed description

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern or Western blot analysis. The total number of thymocytes in homozygous mice is increased, although T cell population subsets are normal. Immature (CD4+CD8+) thymocytes isolated from mutants are less susceptible to activation-induced apoptosis. In an in vitro binding assay activated T lymphocytes do not bind mouse CD30L, a ligand for the gene product.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a portion of the sequence including the second to last exon. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Allele

Symbol: Tnfrsf8^tm1Mak
Name: targeted mutation 1, Tak Mak
MGI accession ID: MGI:1860056
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tnfrsf8
Marker name: tumor necrosis factor receptor superfamily, member 8
Chromosome: 4
Strain of origin: 129P2/OlaHsd
Molecular note: A construct containing a neomycin resistance gene in the antisense direction was used to replace the second last (or second to last) exon. A dish binding assay indicated the absence of functional Tnfrsf8 protein on the surface of spleen cells isolated from homozygous mutant mice.