These Tnfrsf8 knock-out mice exhibit an increased number of thymocytes.
Dr. Tak Mak, University Health Network/Un of Toronto
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Tnfrsf8 | tumor necrosis factor receptor superfamily, member 8 |
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern or Western blot analysis. The total number of thymocytes in homozygous mice is increased, although T cell population subsets are normal. Immature (CD4+CD8+) thymocytes isolated from mutants are less susceptible to activation-induced apoptosis. In an in vitro binding assay activated T lymphocytes do not bind mouse CD30L, a ligand for the gene product.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a portion of the sequence including the second to last exon. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
Allele Name | targeted mutation 1, Tak Mak |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD30 - |
Gene Symbol and Name | Tnfrsf8, tumor necrosis factor receptor superfamily, member 8 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 4 |
Molecular Note | A construct containing a neomycin resistance gene in the antisense direction was used to replace the second last (or second to last) exon. A dish binding assay indicated the absence of functional Tnfrsf8 protein on the surface of spleen cells isolated from homozygous mutant mice. |
Mutations Made By | Dr. Tak Mak, University Health Network/Un of Toronto |
The resulting chimeric animals were crossed to C57BL/6 mice, then backcrossed to the same for 6 generations. Coat color expected from breeding:Black
When using the CD30- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004457 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Tnfrsf8<tm1Mak> |
Frozen Mouse Embryo | B6.129P2-Tnfrsf8<tm1Mak>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Tnfrsf8<tm1Mak>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Tnfrsf8<tm1Mak>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Tnfrsf8<tm1Mak>/J Frozen Embryos | $3373.50 |
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