NOD mice homozygous for the Tcratm1Mjo targeted mutation lack alpha beta T cells, and therefore, are completely protected from diabetes development. Because of the complete elimination of alpha beta T cells, these mice are useful in adoptive transfer experiments (as in Hoglund et al. 1999).
Christophe Benoist, Joslin Diabetes Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tcra | T cell receptor alpha chain |
NOD mice homozygous for the Tcratm1Mjo targeted mutation lack alpha beta T cells, and therefore, are completely protected from diabetes development. Because of the complete elimination of alpha beta T cells, these mice are useful in adoptive transfer experiments (as in Hoglund et al. 1999).
The Tcratm1Mjo targeted mutation was generated in GK129 ES cells (derived from substrain129P2/OlaHsd) via homologous recombination which inserted the neomycin resistance gene under the control of a eukaryotic promoter into the first constant region of the Tcra gene. ES cells carrying the disrupted gene were injected into BALB/c blastocysts, chimeric founders were bred with BALB/c females, and their heterozygous offspring were sibling mated to homozygosity. The Tcratm1Mjo allele on the C;129 mixed background was then transfered to the NOD background and in July 2002 the generation of this strain was N12. (Philpott et al. 1992.)
Allele Name | targeted mutation 1, Michael J Owen |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Calpha-; Tcratm1Phi; TCRalpha; Tcralpha-; TCRalpha KO; TCRalphae- |
Gene Symbol and Name | Tcra, T cell receptor alpha chain |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 14 |
Molecular Note | The Tcra-C element C1 was disrupted by the insertion of a neomycin selection cassette. |
Mutations Made By | Michael Owen, Imperial Cancer Research Fund |
When using the NOD.Tcra mouse strain in a publication, please cite the originating article(s) and include JAX stock #004444 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Tcra<tm1Mjo> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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