NOD.129P2(C)-Tcra^tm1Mjo/DoiJ

Stock number: 004444
Product name: NOD.Tcra
Research areas: Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

NOD mice homozygous for the Tcra^tm1Mjo targeted mutation lack alpha beta T cells, and therefore, are completely protected from diabetes development. Because of the complete elimination of alpha beta T cells, these mice are useful in adoptive transfer experiments (as in Hoglund et al. 1999).

Development

The Tcra^tm1Mjo targeted mutation was generated in GK129 ES cells (derived from substrain129P2/OlaHsd) via homologous recombination which inserted the neomycin resistance gene under the control of a eukaryotic promoter into the first constant region of the Tcra gene. ES cells carrying the disrupted gene were injected into BALB/c blastocysts, chimeric founders were bred with BALB/c females, and their heterozygous offspring were sibling mated to homozygosity. The Tcra^tm1Mjo allele on the C;129 mixed background was then transfered to the NOD background and in July 2002 the generation of this strain was N12. (Philpott et al. 1992.)

Allele

Symbol: Tcra^tm1Mjo
Name: targeted mutation 1, Michael J Owen
MGI accession ID: MGI:2180883
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tcra
Marker name: T cell receptor alpha chain
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: The Tcra-C element C1 was disrupted by the insertion of a neomycin selection cassette.