These Gdf1 knock-out mice exhibit an embryonic and perinatal lethal phenotype and newborn mice display a range of left-right axis defects.
Dr. Sejin Lee, Johns Hopkins Univ. School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Gdf1 | growth differentiation factor 1 |
Mice that are heterozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null mice have an embryonic and perinatal lethal phenotype, most develop past embryonic day 14.5 with two thirds dying at birth and almost all not surviving beyond 48 hours after birth. Homozygous mutant newborn mice display a range of left-right axis defects such as visceral situs inversus, right pulmonary isomerism and cardiac anomalies including abnormal positioning of the aorta and pulmonary artery, and septal defects. Spleen and lungs were malformed in some homozygotes. This mutant mouse strain represents a model that may be useful in studies of situs inversus.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the entire coding region of the targeted allele. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to B6;129 mice.
Allele Name | targeted mutation 1, Se-Jin Lee |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Gdf1- |
Gene Symbol and Name | Gdf1, growth differentiation factor 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 8 |
Molecular Note | The gene was disrupted by replacement of exon 8 with a neomycin resistance cassette via homologous recombination. Homozygous mutant animals were identified by Southern blot analysis. |
Mutations Made By | Dr. Sejin Lee, Johns Hopkins Univ. School of Medicine |
This strain originated on a B6;129 background and is maintained as a heterozygote on the same. Cryo's mating scheme for freeze is Inbred X Homo <4/28/03>.
When using the Gdf1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004425 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gdf1<tm1Sjl> |
Frozen Mouse Embryo | B6;129-Gdf1<tm1Sjl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gdf1<tm1Sjl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gdf1<tm1Sjl>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Gdf1<tm1Sjl>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.