B6;129-Gdf1^tm1Sjl/J

Stock number: 004425
Product name: Gdf1-
Research areas: Developmental Biology Research

Description

These Gdf1 knock-out mice exhibit an embryonic and perinatal lethal phenotype and newborn mice display a range of left-right axis defects.

Detailed description

Mice that are heterozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null mice have an embryonic and perinatal lethal phenotype, most develop past embryonic day 14.5 with two thirds dying at birth and almost all not surviving beyond 48 hours after birth. Homozygous mutant newborn mice display a range of left-right axis defects such as visceral situs inversus, right pulmonary isomerism and cardiac anomalies including abnormal positioning of the aorta and pulmonary artery, and septal defects. Spleen and lungs were malformed in some homozygotes. This mutant mouse strain represents a model that may be useful in studies of situs inversus.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the entire coding region of the targeted allele. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to B6;129 mice.

Allele

Symbol: Gdf1^tm1Sjl
Name: targeted mutation 1, Se-Jin Lee
MGI accession ID: MGI:2181719
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gdf1
Marker name: growth differentiation factor 1
Chromosome: 8
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The gene was disrupted by replacement of exon 8 with a neomycin resistance cassette via homologous recombination. Homozygous mutant animals were identified by Southern blot analysis.