These Ptger2 knock-out mice exhibit slightly elevated baseline systolic blood pressure and reduced fertility in homozygous females.
Richard M. Breyer, Vanderbilt University
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes driven by the mouse phosphoglycerate kinase promoter was used to disrupt sequence of the targeted gene encoding the N-terminal of the protein. The construct was electroporated into 129S6/SvEv derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6 females. Simple sequence length polymorphism (SSLP) analysis confirmed that there are no chromosomal segments derived from 129, except for regions immediately flanking the disrupted locus, proximal chromosome 14. This strain originated on a B6;129 background and has been backcrossed to C57BL/6J for 5 generations (using a speed congenic protocol) to achieve homozygosity.
|Allele Name||targeted mutation 1, Richard M Breyer|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ptger2, prostaglandin E receptor 2 (subtype EP2)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A PGK-neomycin resistance cassette replaced part of the amino terminal coding sequence, including 420 bp from the ATG start codon to codon 140.|
|Mutations Made By|| |
Richard Breyer, Vanderbilt University
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the EP2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004376 in your Materials and Methods section.