These K-Ras4bG12D responder mice express a transgene containing activated rat Kras under the regulation of a tetracycline-responsive promoter element (TRE; tetO).
Dr. Harold E. Varmus, Weill Cornell Medical College
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
Mice that are homozygous for the transgene are viable and fertile. These transgenic mice express activated rat Kras (Kras4bG12D) under the regulation of a tetracycline-responsive promoter element (TRE; tetO). When these mice are mated to strains containing transgenes encoding either rtTA (reverse tetracycline trans-activator protein) or tTA (the tetracycline trans-activator), activated Kras expression in the bitransgenic animals can be induced by administration or withdrawl, respectively, of the tetracycline analog, doxycycline. This strain represents an effective tool for generating tissue specific mutants that would be useful models for studying oncogene activation in human carcinogenesis.
A transgenic construct containing the mutant gene (Kras4bG12D) under the regulation of a tetracycline-responsive promoter element (TRE; tetO), the mp-1 intron and polyadenylation site sequence, and a minimal CMV promoter was injected into FVB/N blastocysts.
Expressed Gene | Kras, KRAS proto-oncogene, GTPase, rat |
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Site of Expression |
Allele Name | transgene insertion 12, Harold E Varmus |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | KE; K-Ras4bG12D responder; K-ras4bG12D; K-Ras4bG12D responder; K-rasG12D-5; TetO-KrasG12D; Tet-op-K-ras; Tet-op-K-RasG12D; TOR; tre-Kras; TRE-KrasG12D |
Gene Symbol and Name | Tg(tetO-Kras2)12Hev, transgene insertion 12, Harold E Varmus |
Gene Synonym(s) | |
Promoter | CMV, cytomegalovirus, human |
Promoter | tetO, tet operator, |
Expressed Gene | Kras, KRAS proto-oncogene, GTPase, rat |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Homozygous transgenic mice are viable and fertile. In conjunction with a second transgene encoding either rtTA (reverse tetracycline trans-activator protein) or tTA (the tetracycline trans-activator), mice carrying Tg(tetO-Kras2)12Hev show tissue-specific expression of the mutated K-ras transgene that may be induced or suppressed by administration of the tetracycline analog, doxycycline. Cessation or initiation of doxycycline treatment in bitransgenic strains containing, respectively, rtTA or tTA, will result in a rapid decrease of expression of the transgene. |
Molecular Note | The transgene expresses the mutant gene Kras4bG12D, under the control of a tetracycline-responsive promoter element (tTA), mp-1 intron polyadenylation site sequence, and a minimal CMV promoter. |
Mutations Made By | Dr. Harold Varmus, Weill Cornell Medical College |
This strain originated on a FVB/N background and has been maintained on the same.
When using the K-Ras4bG12D responder mouse strain in a publication, please cite the originating article(s) and include JAX stock #004375 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(tetO-Kras2)12Hev |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Kras2)12Hev/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Kras2)12Hev/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Kras2)12Hev/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Kras2)12Hev/J Frozen Embryos | $3373.50 |
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