Mice that are homozygous for the targeted mutation are viable, normal in size at birth and do not display any gross physical or behavioral abnormalities. This targeted mutation is X-linked; males bearing the targeted allele display a mutant phenotype. At 15 to 16 weeks of age male mice heterozygous for the mutant allele display increased body weight as compared to wildtype littermates. This mutant mouse strain represents a model that may be useful in studies related to energy metabolism and obesity.
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt exon 2 of the targeted allele. The construct was electroporated into 129X1/SvJ derived Gsi1 embryonic stem (ES) cells. The resulting chimeric animals were backcrossed to C57BL/6 mice.
|Allele Name||targeted mutation 1, James F Battey|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||BRS-3 KO|
|Gene Symbol and Name||Brs3, bombesin-like receptor 3|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exon 2 has been replaced with a neomycin resistance cassette. No transcript from the disrupted gene was detected in mRNA from brains of homozygous mutant mice by reverse transcription followed by PCR analysis (RT-PCR).|
|Mutations Made By|| |
Dr. Lori Hampton, NIDCD-NIH
This strain originated on a 129 background, has been backcrossed for 9 generations on the C57BL/6J background. When held in a live colony, breeder pairs were het female X hemi male.
When using the BRS-3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004366 in your Materials and Methods section.