These Brs3 knock-out mice exhibit a greater than normal body weight.
Dr. James Battey, Jr., National Institutes of Health
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Brs3 | bombesin-like receptor 3 |
Mice that are homozygous for the targeted mutation are viable, normal in size at birth and do not display any gross physical or behavioral abnormalities. This targeted mutation is X-linked; males bearing the targeted allele display a mutant phenotype. At 15 to 16 weeks of age male mice heterozygous for the mutant allele display increased body weight as compared to wildtype littermates. This mutant mouse strain represents a model that may be useful in studies related to energy metabolism and obesity.
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt exon 2 of the targeted allele. The construct was electroporated into 129X1/SvJ derived Gsi1 embryonic stem (ES) cells. The resulting chimeric animals were backcrossed to C57BL/6 mice.
Allele Name | targeted mutation 1, James F Battey |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | BRS-3 KO |
Gene Symbol and Name | Brs3, bombesin-like receptor 3 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | X |
Molecular Note | Exon 2 has been replaced with a neomycin resistance cassette. No transcript from the disrupted gene was detected in mRNA from brains of homozygous mutant mice by reverse transcription followed by PCR analysis (RT-PCR). |
Mutations Made By | Dr. Lori Hampton, NIDCD-NIH |
This strain originated on a 129 background, has been backcrossed for 9 generations on the C57BL/6J background. When held in a live colony, breeder pairs were het female X hemi male.
When using the BRS-3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004366 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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X linked - Heterozygous Females and Wild-type Males for Brs3<tm1Jfb> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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