B6.129X1-Brs3^tm1Jfb/J

Stock number: 004366
Product name: BRS-3 KO
Research areas: Diabetes and Obesity Research, Endocrine Deficiency Research, Internal/Organ Research, Metabolism Research, Neurobiology Research, Research Tools

Description

These Brs3 knock-out mice exhibit a greater than normal body weight.

Detailed description

Mice that are homozygous for the targeted mutation are viable, normal in size at birth and do not display any gross physical or behavioral abnormalities. This targeted mutation is X-linked; males bearing the targeted allele display a mutant phenotype. At 15 to 16 weeks of age male mice heterozygous for the mutant allele display increased body weight as compared to wildtype littermates. This mutant mouse strain represents a model that may be useful in studies related to energy metabolism and obesity.

Development

A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt exon 2 of the targeted allele. The construct was electroporated into 129X1/SvJ derived Gsi1 embryonic stem (ES) cells. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Allele

Symbol: Brs3^tm1Jfb
Name: targeted mutation 1, James F Battey
MGI accession ID: MGI:2657217
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Brs3
Marker name: bombesin-like receptor 3
Chromosome: X
Strain of origin: 129X1/SvJ
Molecular note: Exon 2 has been replaced with a neomycin resistance cassette. No transcript from the disrupted gene was detected in mRNA from brains of homozygous mutant mice by reverse transcription followed by PCR analysis (RT-PCR).