These SR-BI/apoE double knock-out mice exhibit hypercholesterolemia, low body weights, and early mortality.
IMR Colony, The Jackson Laboratory
At birth, mice homozygous for both targeted alleles are viable, normal in size and do not display any gross physical or behavioral abnormalities. All double mutants die between 5 to 8 weeks following birth, exhibiting hypercholesterolemia and significantly lower body weights compared to control mice. Mice display an altered appearance several days before death, displaying ruffled fur, an abnormal gate and sometimes labored breathing. Hearts from double mutants are enlarged, exhibit evidence of myocardial infarction and are functionally impaired. Histological examination reveals extensive coronary artery disease characterized by atherosclerotic plaques with evidence of cholesterol clefts and fibrin deposition. As with other Scarb1 homozygous null mutants, females are infertile while the fertility of homozygous males remains intact. This double mutant mouse strain offers a model that may be a useful tool in studies related to coronary heart disease.
Please see strain entries 002052 and 003379 for details regarding the construction of each mutant allele.
|Allele Name||targeted mutation 1, University of North Carolina|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||APOE KO; AopE(-); ApoE-KO; Apoetm1Un; apoE-; apoE0; epsilon-; mE-; mEKO|
|Gene Symbol and Name||Apoe, apolipoprotein E|
|Gene Synonym(s)||AD2; AI255918; APO-E; APOEA; ApoE4; LDLCQ5; LPG; expressed sequence AI255918|
|Strain of Origin||129P2/OlaHsd|
|General Note||Phenotypic Similarity to Human Syndrome: Coronary Artery Disease (CAD) in mice homozygous for Apoe tm1Unc and Scarb1 tm1Kri on a mixed 129, BALB/c and C57BL/6 background (J:201999) |
Phenotypic Similarity to Human Syndrome: Metabolic Syndrome in mice homozygous for Apoetm1Unc and Cyp19a1tm1Esi (J:184647)
Phenotypic Similarity to Human Syndrome: Metabolic Syndrome in mice homozygous for Apoetm1Unc and heterozygous for Ay and a (J:177084)
|Molecular Note||Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE.|
|Mutations Made By|| |
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
|Allele Name||targeted mutation 1, Monty Krieger|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||SR-BI; SR-BI KO; SR-BI-; Sr-b1-; srb1-; srbI-|
|Gene Symbol and Name||Scarb1, scavenger receptor class B, member 1|
|Gene Synonym(s)||AI120173; CD36L1; CLA-1; CLA1; Cd36l1; Chohd1; Chohd1; Cla-1; D5Ertd460e; D5Ertd460e; DNA segment, Chr 5, ERATO Doi 460, expressed; HDL QTL 1; HDLQTL6; Hdlq1; Hdlq1; Hlb398; Hlb398; SR-B1; SR-BI; SRB1; SRBI; Srb1; Srb1; dominant hypercholesterolemia 1; expressed sequence AI120173; heart, lung and blood 398; scavenger receptor class B1|
|Strain of Origin||129S2/SvPas|
|General Note||Phenotypic Similarity to Human Syndrome: Coronary Artery Disease (CAD) in mice homozygous for Apoe tm1Unc and Scarb1 tm1Kri on a mixed 129, BALB/c and C57BL/6 background (J:201999)|
|Molecular Note||Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette.|
|Mutations Made By|| |
Dr. Monty Krieger, Massachusetts Institute of Technology
When maintaining a live colony, this strain is maintained by mating Apoe/Apoe Scarb1/+ x Apoe/Apoe +/+ or reciprocal. Note: mice homozygous for both null alleles die between 5 to 8 weeks.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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