These SR-BI/apoE double knock-out mice exhibit hypercholesterolemia, low body weights, and early mortality.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Apoe | apolipoprotein E |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Scarb1 | scavenger receptor class B, member 1 |
At birth, mice homozygous for both targeted alleles are viable, normal in size and do not display any gross physical or behavioral abnormalities. All double mutants die between 5 to 8 weeks following birth, exhibiting hypercholesterolemia and significantly lower body weights compared to control mice. Mice display an altered appearance several days before death, displaying ruffled fur, an abnormal gate and sometimes labored breathing. Hearts from double mutants are enlarged, exhibit evidence of myocardial infarction and are functionally impaired. Histological examination reveals extensive coronary artery disease characterized by atherosclerotic plaques with evidence of cholesterol clefts and fibrin deposition. As with other Scarb1 homozygous null mutants, females are infertile while the fertility of homozygous males remains intact. This double mutant mouse strain offers a model that may be a useful tool in studies related to coronary heart disease.
Please see strain entries 002052 and 003379 for details regarding the construction of each mutant allele.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | AopE(-); apoE-; APOE KO; Apoetm1Un; apoE0; ApoE-KO; EKO; epsilon-; mE-; mEKO |
Gene Symbol and Name | Apoe, apolipoprotein E |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. |
Mutations Made By | Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill |
Allele Name | targeted mutation 1, Monty Krieger |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | srb1-; Sr-b1-; SR-BI; SR-BI KO; SR-BI-; srbI- |
Gene Symbol and Name | Scarb1, scavenger receptor class B, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 5 |
Molecular Note | Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette. |
Mutations Made By | Dr. Monty Krieger, Massachusetts Institute of Technology |
When maintaining a live colony, this strain is maintained by mating Apoe/Apoe Scarb1/+ x Apoe/Apoe +/+ or reciprocal. Note: mice homozygous for both null alleles die between 5 to 8 weeks.
Of note, a similar strain, Srb1deltaCT/Hypo E (Stock No. 032063), is viable and fertile as double homozygotes.
When using the SR-BI/apoE double KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004362 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Scarb1<tm1Kri>, Homozygous for Apoe<tm1Unc> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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