These NOD.Rag1null.AI4bTg mice express a transgene containing a rearranged Tcrb derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4, and are incapable of rearranging endogenous T cell receptor genes as a result of the nonfunctional Rag1tm1Mom allele.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Allele Type |
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Transgenic (Inserted expressed sequence) |
The rearranged Tcrb transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The presence of the nonfunctional Rag1tm1Mom allele renders mice incapable of rearranging endogenous T cell receptor genes. When crossed with strain NOD.Cg-Tg(TcraAI4)1Dvs-Rag1tm1Mom/DvsJ (004347), the resulting animals that carry both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM). Additionally, transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to the earliest phase of IDDM development. Homozygous mice are viable and fertile.
A transgenic construct containing a Tcrb-V2/Tcrb-D/Tcrb-J cDNA segment was injected into NOD/Lt zygotes. An outcross-intercross strategy was employed to introduce an inactivated Rag1 allele from an N11 congenic stock, NOD.129S7(B6)-Rag1tm1Mom/J mice (strain 003729).
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion 1, David V Serreze |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | AI4bTg |
Gene Symbol and Name | Tg(TcrbAI4)1Dvs, transgene insertion 1, David V Serreze |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
General Note | In conjunction with Rag1tm1Mom
|
Molecular Note | The rearranged Tcrb transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgenic construct contains a Tcrb-V2/Tcrb-D/Tcrb-J cDNA segment. |
Mutations Made By | Dr. David Serreze, The Jackson Laboratory |
These mice are maintained homozygous for Rag1 and are severely immunodeficient, so specific pathogen-free (SPF) conditions are recommended. This strain is maintaned through mating Rag1-/Rag1-, Tg/? x Rag1-/Rag1-, Tg/?. Genotypes available are Rag1-/Rag1-, Tg? or Rag1-/Rag1-, +/+. breeder pairs available: Rag1-/Rag1-, Tg/? x Rag1-/Rag1-, Tg/?. Affected mutant is Rag1-/Rag1-, Tg/?
When using the NOD.Rag1null.AI4bTg mouse strain in a publication, please cite the originating article(s) and include JAX stock #004348 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Rag1<tm1Mom>, Heterozygous for Tg(TcrbAI4)1Dvs |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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