These mice are characterized by pancreatic beta cells that express a rat insulin promoter (Rip) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. They are an excellent source of MHC class I positive, CD80 transgene-expressing islet cells, a potent antigenic reagent for propagating diabetogenic CD8+ T lymphocytes derived from NOD mice in vitro. The presence of the Prkdcscid allele eliminates the possibility that isolated islet cells will introduce contaminating T lymphocytes onto cultures.

Mice homozygous for the Prkdcscid mutation and hemizygous for the Tg(Ins2-CD80)3B7Flv transgene are viable and fertile. In 2016, it is the experience of The Jackson Laboratory Repository that bi-weekly treatment of medicated water (described below) greatly improves colony health / is required for colony to thrive. 

NOD mice homozygous for the Prkdcscid mutation are characterized by an absence of functional T cells and B cells - resulting in severe immunodeficiency.
As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If mice homozygous for Prkdcscid are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.

These NOD-scid.RIPB7 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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