NOD.Cg-Prkdc^scid Tg(Ins2-CD80)3B7Flv/DvsJ

Stock number: 004346
Product name: NOD-scid.RIPB7
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These NOD-scid.RIPB7 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule.

Detailed description

These mice are characterized by pancreatic beta cells that express a rat insulin promoter (Rip) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. They are an excellent source of MHC class I positive, CD80 transgene-expressing islet cells, a potent antigenic reagent for propagating diabetogenic CD8+ T lymphocytes derived from NOD mice in vitro. The presence of the Prkdc^scid allele eliminates the possibility that isolated islet cells will introduce contaminating T lymphocytes onto cultures.

Mice homozygous for the Prkdc^scid mutation and hemizygous for the Tg(Ins2-CD80)3B7Flv transgene are viable and fertile. In 2016, it is the experience of The Jackson Laboratory Repository that bi-weekly treatment of medicated water (described below) greatly improves colony health / is required for colony to thrive.

NOD mice homozygous for the Prkdc^scid mutation are characterized by an absence of functional T cells and B cells - resulting in severe immunodeficiency. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If mice homozygous for Prkdc^scid are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.

Development

A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (Rip) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice, and partially transferred to the NOD background through five backcross generations. Mice with the partial NOD background were obtained from the Flavell laboratory and crossed to NOD.CB-17-Prkdc^scid/J (Jax strain 001303) for eight backcross generations by Dr. David Serreze, The Jackson Laboratory. Marker assistance was employed in backcrossing protocol.

Allele

Symbol: Tg(Ins2-CD80)3B7Flv
Name: transgene insertion 3B7, Richard Flavell
MGI accession ID: MGI:2682826
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Ins2-CD80)3B7Flv
Marker name: transgene insertion 3B7, Richard Flavell
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/Ca)F2
Expressed genes: CD80,CD80 molecule,human
Molecular note: The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2).
General note: Mice carrying this transgene that also are homozygous for Prkdc^scid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).