Overview

Also Known As:NOD-scid.RIPB7

These NOD-scid.RIPB7 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule.

Donating Investigator

Dr. David Serreze, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

Research Applications

Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Diabetes and Obesity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These mice are characterized by pancreatic beta cells that express a rat insulin promoter (Rip) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. They are an excellent source of MHC class I positive, CD80 transgene-expressing islet cells, a potent antigenic reagent for propagating diabetogenic CD8+ T lymphocytes derived from NOD mice in vitro. The presence of the Prkdc^scid allele eliminates the possibility that isolated islet cells will introduce contaminating T lymphocytes onto cultures.

Mice homozygous for the Prkdc^scid mutation and hemizygous for the Tg(Ins2-CD80)3B7Flv transgene are viable and fertile. In 2016, it is the experience of The Jackson Laboratory Repository that bi-weekly treatment of medicated water (described below) greatly improves colony health / is required for colony to thrive.

NOD mice homozygous for the Prkdc^scid mutation are characterized by an absence of functional T cells and B cells - resulting in severe immunodeficiency.
As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If mice homozygous for Prkdc^scid are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.

Development

A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (Rip) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice, and partially transferred to the NOD background through five backcross generations. Mice with the partial NOD background were obtained from the Flavell laboratory and crossed to NOD.CB-17-Prkdc^scid/J (Jax strain 001303) for eight backcross generations by Dr. David Serreze, The Jackson Laboratory. Marker assistance was employed in backcrossing protocol.

Expression Data

Expressed Gene	CD80, CD80 molecule, human
Site of Expression

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

DiLorenzo TP; Graser RT; Ono T; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 1998. Major histocompatibility complex class I-restricted T cells are required for all but the end stages of diabetes development in nonobese diabetic mice and use a prevalent T cell receptor alpha chain gene rearrangement. Proc Natl Acad Sci U S A 95(21):12538-43PubMed: 9770521 MGI: J:50360

View All References

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Tg(Ins2-CD80)3B7Flv

Allele Symbol: Tg(Ins2-CD80)3B7Flv

Allele Name	transgene insertion 3B7, Richard Flavell
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	RIP-B7; RIP-B7.1; RIP-B7-1; RIP-CD80
Gene Symbol and Name	Tg(Ins2-CD80)3B7Flv, transgene insertion 3B7, Richard Flavell
Gene Synonym(s)
Promoter	Ins2, insulin 2, rat
Expressed Gene	CD80, CD80 molecule, human
Strain of Origin	(C57BL/6 x CBA/Ca)F2
Chromosome	UN
General Note	Mice carrying this transgene that also are homozygous for Prkdc^scid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule.
Molecular Note	The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2).
Mutations Made By	Dr. Richard Flavell, Yale University School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B and T cell deficiency
Diabetes and Obesity Research
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD Congenics with Mutations Affecting Immunocompetence
  - NOD Transgenics

Mammalian Phenotype Terms by Genotype

References

DiLorenzo TP; Graser RT; Ono T; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 1998. Major histocompatibility complex class I-restricted T cells are required for all but the end stages of diabetes development in nonobese diabetic mice and use a prevalent T cell receptor alpha chain gene rearrangement. Proc Natl Acad Sci U S A 95(21):12538-43PubMed: 9770521 MGI: J:50360

Additional References

Guerder S; Picarella DE; Linsley PS; Flavell RA. 1994. Costimulator B7-1 confers antigen-presenting-cell function to parenchymal tissue and in conjunction with tumor necrosis factor alpha leads to autoimmunity in transgenic mice. Proc Natl Acad Sci U S A 91(11):5138-42PubMed: 7515187 MGI: J:88250
Wong S; Guerder S; Visintin I; Reich EP; Swenson KE; Flavell RA; Janeway CA Jr. 1995. Expression of the co-stimulator molecule B7-1 in pancreatic beta-cells accelerates diabetes in the NOD mouse. Diabetes 44(3):326-9PubMed: 7533734 MGI: J:26618

Additional - Prkdc^scid related

Additional - Tg(Ins2-CD80)3B7Flv related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(Ins2-CD80)3B7Flv
- QPCR:Tg(Ins2-CD80)3B7Flv
- Probe:Tg(Ins2-CD80)3B7Flv
- Genotyping resources and troubleshooting
Breeding Considerations

Mice homozygous for the Prkdc^scid mutation and hemizygous for the Tg(Ins2-CD80)3B7Flv transgene are viable and fertile. As of 2016, it is the experience of The Jackson Laboratory Repository that bi-weekly treatment of medicated water (described below) greatly improves colony health and is required for colony to thrive.
NOD mice homozygous for the Prkdc^scid mutation are characterized by an absence of functional T cells and B cells - resulting in severe immunodeficiency. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If mice homozygous for Prkdc^scid are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.
The Jackson Laboratory mating system - Prkdc^scid/ Prkdc^scid, Tg/? XPrkdc^scid/Prkdc^scid, Tg/?
Genotypes available - Prkdc^scid/Prkdc^scid, Tg/? and Prkdc^scid/Prkdc^scid, +/+
Affected mutant - Prkdc^scid/ Prkdc^scid/, Tg/?
Breeder pairs-Prkdc^scid/ Prkdc^scid/, Tg/? x Prkdc^scid/ Prkdc^scid/, Tg/?
- Additional Breeding and Husbandry Support
Appearance
- albino, pink eyed
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD-scid.RIPB7 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004346 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Homozygous for Prkdc<scid>,Homozygous or Hemizygous or Non carrier for Tg(Ins2-CD80)3B7Flv

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NOD-scid.RIPB7

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Prkdcscid

Allele Symbol: Prkdcscid

Tg(Ins2-CD80)3B7Flv

Allele Symbol: Tg(Ins2-CD80)3B7Flv

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Prkdcscid related

Additional - Tg(Ins2-CD80)3B7Flv related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

Genotype: Prkdc^scid related

Additional - Prkdc^scid related