This chromosome 4 congenic stock, commonly referred to as NOD.B10-Idd9, Line 905, contains the C57BL/10SnJ derived Idd9 region, 119Mb through 150Mb, introgressed into the NOD/MrkTac background and exhibits significantly reduced diabetes incidence. This strain may be useful for positional mapping of the Idd9 sub-loci and studying candidate genes within the Idd9 region.
Dr. David Serreze, The Jackson Laboratory
Linda Wicker, University of Cambridge, UK
This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd9, line R905 contains a 31Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 4 and is defined within markers D4Mit258 (119Mb) and D4Mit42 (150Mb). The C57BL/10SnJ genomic segment includes Idd9.1, Idd9.2 and Idd59.3. Line 905 replaces and exhibits the same phenotype as line R28, which had a slight larger congenic interval. Congenic females and males are significantly protected from diabetes (less than 10% diabetic by 220 days of age), yet histological evaluation of 3 and 7 to9-month old females and males showed mild to extensive insulitis and were indistinguishable from the pancreas of NOD controls. When compared with NOD controls, insulin autoantibodies (IAA) are essentially the same at 13-15 weeks of age.
This strain may be useful for positional mapping of the Idd9 sub-loci and studying candidate genes within the Idd9 region.
This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/10SnJ (Stock No. 000666) followed by 13 additional crosses to NOD (N14) with selection for chromosome 4, Idd9 markers. Siblings carrying the C57BL/10SnJ derived segments denoted by markers D4Mit258 (119Mb) through D4Mit42 (150Mb) were intercrossed to make homozygous. This chromosome 4 region contains Idd9.1, Idd9.2 and Idd9.3. NCBI based 5kSNP Chip Data identified no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.
|Gene Symbol and Name||Idd9, insulin dependent diabetes susceptibility 9|
|Strain of Origin||C57BL/10SnJ|
|Molecular Note||This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.|