Jackson Laboratory SEARCH FOR MICE
NOD/ShiLt-Tg(TcraAI4)1Dvs
Stock No: 004334
  • Coisogenic
  • Transgenic
Contact Customer Service
Contact Customer Service
  • Email
  • Download PDF
  • Print
  • Help
  • Overview
  • Details
    • Detailed Description
    • Development
    • Expression Data
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
  • Technical Support
    • Genotyping Protocols
    • Appearance
    • Citation
    • Animal Health Reports
    • Contact Technical Support
  • Pricing & Availability
    • Price
    • Payment Terms
  • Terms Of Use
  • Related Strains

Overview

These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a Kd (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM).

Read More +

Genetic overview

Genetic Background Generation
N6F46
(2017-10-13 00:00:00)

Tg(TcraAI4)1Dvs

Allele Type
Transgenic (Inserted expressed sequence)
View Genetics

Research Applications

  • Research Tools
  • Immunology, Inflammation and Autoimmunity Research
View All Research Applications

Base Price

Starting at:

View Price List

Details

Detailed Description

These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a Kd (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM). In combination with NOD/ShiLt-Tg(TcrbAI4)1Dvs (Stock No. 004335) the AI4 clonotype accelerates development of IDDM. These mice may be useful for studying CD8 T cells and IDDM development.

Development

The transgene encoding the TCR alpha chain from clone AI4 was injected into NOD/Lt zygotes. The strain has been maintained by sibling mating carriers. (Graser et al., 2000.)

Expression Data

Expressed Gene Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression

Genetics

Tg(TcraAI4)1Dvs

Allele Symbol: Tg(TcraAI4)1Dvs

Allele Name transgene insertion 1, David V Serreze
Allele Type Transgenic (Inserted expressed sequence)
Allele Synonym(s) AI4aTg
Gene Symbol and Name Tg(TcraAI4)1Dvs, transgene insertion 1, David V Serreze
Gene Synonym(s)
PromoterTcra, T cell receptor alpha chain, mouse, laboratory
Expressed GeneTcra, T cell receptor alpha chain, mouse, laboratory
Strain of Origin NOD/ShiLt
Chromosome 1
General Note In conjunction with Rag1tm1Mom
    transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).
    homozygous mice are viable and fertile
Molecular Note The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment.
Mutations Made By

Dr. David Serreze, The Jackson Laboratory

Disease/Phenotype

Disease Terms

Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.

  • type 1 diabetes mellitus

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

  • Research Tools
    • Immunology, Inflammation and Autoimmunity Research
      • T Cell Receptor Transgenics
  • Immunology, Inflammation and Autoimmunity Research
    • Autoimmunity

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tg(TcraAI4)1Dvs/0
NOD-Tg(TcraAI4)1Dvs

hematopoietic system phenotype

  • abnormal CD4-positive, alpha beta T cell morphology
    • T cells expressing the transgene comprise ~30% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
    • (MGI Ref ID J:93553)

immune system phenotype

  • abnormal CD4-positive, alpha beta T cell morphology
    • T cells expressing the transgene comprise ~30% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
    • (MGI Ref ID J:93553)
  • increased susceptibility to autoimmune diabetes
    • female NOD mice carrying this transgene develop IDDM at a slightly but not significantly accelerated rate compared to nontransgenic NOD controls
    • (MGI Ref ID J:93553)

References

Additional - Tg(TcraAI4)1Dvs related

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • QPCR:Tg(TcraAI4)1Dvs-qPCR
    • Standard PCR:Tg(TcraA14)
    • Standard PCR:Tg(TcraA14)
    • Genotyping resources and troubleshooting
  • Appearance

    • albino, pink eyed
      Related Genotype: A/A Tyrc/Tyrc
  • Citation

    When using the NOD/ShiLt-Tg(TcraAI4)1Dvs mouse strain in a publication, please cite the originating article(s) and include JAX stock #004334 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

B5 (Low)

Pricing & Availability

Availability Varies
  • Domestic
  • International

Live Mouse

Age Genotype Price
weeks

Cryorecovery - Pricing

Service/Product Description Price
Please inquire

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470
Email: TechTran@jax.org

Related Strains

  • All
  • By Allele
  • By Gene
  • By Collection
View All Strains
There are no related strains using this filter.
▲
  • Stock No: |
    Related By:
▼
FEEDBACK
Did you find what you were looking for?

What information were you hoping to find through your search?

How easy was it to find what you were looking for?

We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.

Please Enter a Valid Email Address

Skip

Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.