NOD/ShiLt-Tg(TcraAI4)1Dvs

Stock number: 004334
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a K^d (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM).

Detailed description

These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a K^d (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM). In combination with NOD/ShiLt-Tg(TcrbAI4)1Dvs (Stock No. 004335) the AI4 clonotype accelerates development of IDDM. These mice may be useful for studying CD8 T cells and IDDM development.

Development

The transgene encoding the TCR alpha chain from clone AI4 was injected into NOD/Lt zygotes. The strain has been maintained by sibling mating carriers. (Graser et al., 2000.)

Allele

Symbol: Tg(TcraAI4)1Dvs
Name: transgene insertion 1, David V Serreze
MGI accession ID: MGI:3057179
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcraAI4)1Dvs
Marker name: transgene insertion 1, David V Serreze
Chromosome: 1
Strain of origin: NOD/ShiLt
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment.
General note: In conjunction with Rag1^tm1Mom

transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).

homozygous mice are viable and fertile