NOD/ShiLt-Tg(TcraAI4)1Dvs

Stock number: 004334
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This strain has been discontinued. Tg(TcraAI4)1Dvs can be obtained from Stock No. 004347, Stock No. 004554, or Stock No. 006436.

Detailed description

These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a K^d (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM). In combination with NOD/ShiLt-Tg(TcrbAI4)1Dvs (Stock No. 004335) the AI4 clonotype accelerates development of IDDM. These mice may be useful for studying CD8 T cells and IDDM development.

Development

The transgene encoding the TCR alpha chain from clone AI4 was injected into NOD/Lt zygotes. The strain has been maintained by sibling mating carriers and is now homozygous for this transgene. (Graser et al., 2000.)

Allele

Symbol: Tg(TcraAI4)1Dvs
Name: transgene insertion 1, David V Serreze
MGI accession ID: MGI:3057179
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: AI4aTg
Marker symbol: Tg(TcraAI4)1Dvs
Marker name: transgene insertion 1, David V Serreze
Marker synonyms: AI4aTg
Chromosome: 1
Strain of origin: NOD/ShiLt
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment.
General note: In conjunction with Rag1^tm1Mom

transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).

homozygous mice are viable and fertile