These floxed mutant mice possess loxP sites flanking the second transmembrane domain of the Gabra1 gene. This strain may be useful for generating conditional mutations in applications related to development of cerebellar neurons.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Gabra1 | gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 |
These mice possess loxP sites flanking the second transmembrane domain of the gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 (Gabra1) gene. GABA is an inhibitory neurotransmitter in the nervous system which helps regulate neuronal excitability. Mice that are homozygous for this floxed Gabra1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of Gabra1.
For example, when these mice are bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) expressing cre in all tissues, the resulting double mutant mice exhibit sex-dependent decreased survival after P19, decreased body mass, EEG spike-wave discharges, and seizures. Heterozygotes display an intermediate decrease in body mass and survival.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
A targeting vector was constructed containing a selectable marker cassette (Neo/Tk) flanked by loxP sites. The loxP-flanked cassette was placed approximately 0.6 kb 5' of an exon encoding nucleotides 1307-1509 of the gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 (Gabra1) gene. A third loxP- site was placed approximately 0.8 kb 3' of the same exon. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a supercoiled cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exon (F) were injected in C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and subsequently made homozygous (F/F). The homozygotes were bred to an actin-Cre general deleter on an FVB/N genetic background. Selected mice that were heterozygous for the flanked exon (F/+) and lacked the cre transgene were obtained and crossed to C57BL/6 animals.
Allele Name | targeted mutation 1, Gregg E Homanics |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Gabra1, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A loxP flanked neomycin and thymidine kinase cassette was inserted 5' to sequences corresponding to nucleotides 1307-1509, and a single loxP site was inserted 3' to these sequences. The loxP flanked neomycin selection cassette was removed in ES cells by transient Cre recombinase expression prior to the production of chimeric mice, leaving a single loxP site in place of the cassette. |
Mutations Made By | Gregg Homanics, University of Pittsburgh |
This strain originated on a B6;129 background, was crossed to a pure FVB/N mouse once (a Cre strain) and then crossed to C57BL/6 animals for at least 6 generations (2/02).Coat color expected from breeding:Black
When using the Gabra-1 floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #004318 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Gabra1<tm1Geh> |
Frozen Mouse Embryo | B6.129(FVB)-Gabra1<tm1Geh>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129(FVB)-Gabra1<tm1Geh>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129(FVB)-Gabra1<tm1Geh>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129(FVB)-Gabra1<tm1Geh>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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