NOD.ALR-(D17Mit16-D17Mit10)/LtJ

Stock number: 004309
Product name: NOD-Idd16a^ALR
Strain synonyms: NOD.ALR-DR1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These mice carry the ALR H2^gx complex and insulin dependent diabetes susceptibility locus 16, Idd16a^ALR from ALR/LtJ and exhibit suppression of diabetes incidence.

Detailed description

This allelic congenic strain commonly called D.R1, carries a defined segment of Chr. 17 that includes the ALR H2^gx complex and insulin dependent diabetes susceptibility locus 16, Idd16a^ALR. At 35 weeks of age diabetes incidence in this NOD.ALR strain is significantly suppressed with about 60% of the females and less than 20% of the males diabetic as compared to NOD/ShiLt in which 100% of the females and nearly 70% of the males were diabetic. Histological exam of the pancreas of 40-week old pre-diabetic animals showed widespread intra-islet insulitis. This strain is useful for identifying candidate genes and dissecting the role of Idd16a in autoimmune diabetes.

Development

ALR/LtJ-derived portions of the Idd16 locus were introgressed onto the genetic background of NOD/LtJ. Parental strain ALR/LtJ is resistant to insulin-dependent (type 1) diabetes whereas parental strain NOD/LtJ is susceptible. This strain was produced by mating mice from NOD/ShiLt to ALR/Lt followed by 10 additional crosses to NOD/ShiLt with selection for H2^gx complex at each generation. Siblings carrying the ALR/Lt derived D17Mit16 through D17Mit10 were intercrossed to make homozygous. In 2007, the T1DR received this strain at generation N10F17.

Allele

Symbol: Idd16.1^ALR/LtJ
Name: ALR/LtJ
MGI accession ID: MGI:3579888
Generation method: QTL
Marker symbol: Idd16.1
Marker name: insulin dependent diabetes susceptibility 16.1
Chromosome: 17
Strain of origin: ALR/LtJ
Molecular note: This allele confers resistance to type I diabetes mellitus compared to NOD/LtJ.
General note: This allele confers slower insulitis progression.

Allele

MGI accession ID: MGI:90801
Marker symbol: D17Mit16
Marker name: DNA segment, Chr 17, Massachusetts Institute of Technology 16
Chromosome: 17

Allele

MGI accession ID: MGI:90738
Marker symbol: D17Mit10
Marker name: DNA segment, Chr 17, Massachusetts Institute of Technology 10
Chromosome: 17

Allele

MGI accession ID: MGI:96400
Marker symbol: Idd1
Marker name: insulin dependent diabetes susceptibility 1
Chromosome: 17