NOD.129S4(B6)-Art2a^tm1Fkn Art2b^tm1Fkn/Lt

Stock number: 004307

Description

This strain is discontinued, please refer to NOD.129S4(B6)-Art2a^tm1Fkn Art2b^tm1Fkn/LtJ Stock No. 008252.

Development

The Art2a and Art2b genes were disrupted via homologous recombination in 129S4/SvJae-derived J1 ES cells. Most of exon 3, the major coding exon, of Art2a was replaced with a neomycin resistance cassette and this Art2a^tm1Fkn-bearing cell line was then transfected with a construct which replaced most of the major coding exon of Art2b with a hygromycin resistance cassette. The resulting ES cell with two targeted mutations was injected into blastocysts and a resulting founder was bred once with C57BL/6J and the offspring were intercrossed. F3 mice were sent from Dr. Nigel Killeen to Dr. Edward Leiter at The Jackson Laboratory and backcrossed onto the NOD/Lt background. In 2004 this strain reached N7F2.

Allele

Symbol: Art2b^tm1Fkn
Name: targeted mutation 1, Friedrich Koch-Nolte
MGI accession ID: MGI:2388827
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Art2b
Marker name: ADP-ribosyltransferase 2b
Chromosome: 7
Strain of origin: 129S4/SvJae
Molecular note: The coding region was disrupted by a hygromycin selection cassette inserted by homologous recombination. This construct was used to transfect mice in which the Art2a^tm1Fkn allele had already been created Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes. FACS analysis of thymocytes and lymphocytes indicated a lack of protein expression on the cell surface.
General note: The targeting vector was electroporated into J1 ES cells carrying Art2a^tm1Fkn.

Allele

Symbol: Art2a^tm1Fkn
Name: targeted mutation 1, Friedrich Koch-Noltet
MGI accession ID: MGI:2388822
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Art2a
Marker name: ADP-ribosyltransferase 2a
Chromosome: 7
Strain of origin: 129S4/SvJae
Molecular note: The coding region was disrupted by a neomycin selection cassette inserted by homologous recombination. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes.
General note: Successfully targeted ES cells were subsequently targeted with the construct for the Art2b allele.