Overview

This strain is discontinued, please refer to NOD.129S4(B6)-Art2a^tm1Fkn Art2b^tm1Fkn/LtJ Stock No. 008252.

Genetic overview

Genetic Background	Generation

Art2a^tm1Fkn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Art2a	ADP-ribosyltransferase 2a

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Art2b	ADP-ribosyltransferase 2b

Research Applications

View All Research Applications

Details

Development

The Art2a and Art2b genes were disrupted via homologous recombination in 129S4/SvJae-derived J1 ES cells. Most of exon 3, the major coding exon, of Art2a was replaced with a neomycin resistance cassette and this Art2a^tm1Fkn-bearing cell line was then transfected with a construct which replaced most of the major coding exon of Art2b with a hygromycin resistance cassette. The resulting ES cell with two targeted mutations was injected into blastocysts and a resulting founder was bred once with C57BL/6J and the offspring were intercrossed. F3 mice were sent from Dr. Nigel Killeen to Dr. Edward Leiter at The Jackson Laboratory and backcrossed onto the NOD/Lt background. In 2004 this strain reached N7F2.

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Genetics

Art2a^tm1Fkn

Allele Symbol: Art2a^tm1Fkn

Allele Name	targeted mutation 1, Friedrich Koch-Noltet
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	ART2.1-
Gene Symbol and Name	Art2a, ADP-ribosyltransferase 2a
Gene Synonym(s)
Strain of Origin	129S4/SvJae
Chromosome	7
General Note	Successfully targeted ES cells were subsequently targeted with the construct for the Art2b allele.
Molecular Note	The coding region was disrupted by a neomycin selection cassette inserted by homologous recombination. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes.

Art2b^tm1Fkn

Allele Symbol: Art2b^tm1Fkn

Allele Name	targeted mutation 1, Friedrich Koch-Nolte
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	ART2.2-
Gene Symbol and Name	Art2b, ADP-ribosyltransferase 2b
Gene Synonym(s)
Strain of Origin	129S4/SvJae
Chromosome	7
General Note	The targeting vector was electroporated into J1 ES cells carrying Art2a^tm1Fkn.
Molecular Note	The coding region was disrupted by a hygromycin selection cassette inserted by homologous recombination. This construct was used to transfect mice in which the Art2a^tm1Fkn allele had already been created Transcript was undetected in homozygous mutant mice by RT-PCR analysis of T cells isolated from lymph nodes. FACS analysis of thymocytes and lymphocytes indicated a lack of protein expression on the cell surface.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Art2a^tm1Fkn/Art2a^tm1Fkn Art2b^tm1Fkn/Art2b^tm1Fkn
NOD.129S4(B6)-Art2a Art2b/Lt

cellular phenotype

decreased T cell apoptosis
- CD4+ and CD8+ T cells are completely resistant to NAD-mediated apoptosis in Art2-deficient NOD mice compared to wild-type NOD mice which exhibit T cell apoptosis in a dose-dependent manner compared to Art2-intact NOD mice

hematopoietic system phenotype

decreased T cell apoptosis
- CD4+ and CD8+ T cells are completely resistant to NAD-mediated apoptosis in Art2-deficient NOD mice compared to wild-type NOD mice which exhibit T cell apoptosis in a dose-dependent manner compared to Art2-intact NOD mice

immune system phenotype

decreased T cell apoptosis
- CD4+ and CD8+ T cells are completely resistant to NAD-mediated apoptosis in Art2-deficient NOD mice compared to wild-type NOD mice which exhibit T cell apoptosis in a dose-dependent manner compared to Art2-intact NOD mice

References

Additional - Art2a^tm1Fkn related

Additional - Art2b^tm1Fkn related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino, pink eyed
  Related Genotype: A/A Tyr^c/Tyr^c

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Genetic overview

Research Applications

Development

Control Suggestions

Additional Information

Art2atm1Fkn

Allele Symbol: Art2atm1Fkn

Art2btm1Fkn

Allele Symbol: Art2btm1Fkn

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Art2atm1Fkn/Art2atm1Fkn Art2btm1Fkn/Art2btm1FknNOD.129S4(B6)-Art2a Art2b/Lt

cellular phenotype

hematopoietic system phenotype

immune system phenotype

References

Additional - Art2atm1Fkn related

Additional - Art2btm1Fkn related

Genotyping Protocols

Appearance

Terms Of Use

Licensing Information

Art2a^tm1Fkn

Allele Symbol: Art2a^tm1Fkn

Art2b^tm1Fkn

Allele Symbol: Art2b^tm1Fkn

Genotype: Art2a^tm1Fkn/Art2a^tm1Fkn Art2b^tm1Fkn/Art2b^tm1Fkn
NOD.129S4(B6)-Art2a Art2b/Lt

Additional - Art2a^tm1Fkn related

Additional - Art2b^tm1Fkn related