These F9 knock-out mice exhibit curtailed clotting activity with spontaneous hemorrhage in footpad tissues and subcutaneous hemorrhages.
Darrel W. Stafford, University of North Carolina
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | F9 | coagulation factor IX |
Mice that are homozygous for this targeted mutation are viable, fertile and normal in size. As this mutation is X-linked, males bearing the targeted allele display a mutant phenotype similar to that seen in homozygous females. Gene product activity, as measured by clotting activity using an activated partial thromboplastin time (APTT) Factor IX assay, was reduced to 8% of normal. Mutant mice experience spontaneous bleeding that can lead to swelling of the top of the feet or the footpads. Sudden death due to massive internal hemorrhaging can occur as a result of normal fighting in the cage. Tail cut wounds must be cauterized to prevent blood loss and death in mutant mice. This mutant mouse strain represents a model that may be useful in studies related to gene therapy methods and function of factor IX mutations.
A targeting vector containing a neomycin resistance gene, herpes simplex virus thymidine kinase gene and a hypoxanthine phosphoribosyl transferase minigene (partially deleted and nonfunctional) was used to disrupt exons 1-3 of the targeted gene. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were backcrossed to C57BL/6J mice for 10 generations.
Allele Name | targeted mutation 1, Darrel W Stafford |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | factor IX-knockout; FIX(-); HB |
Gene Symbol and Name | F9, coagulation factor IX |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | Exons 1, 2 and 3 are replaced by a neomycin/hprt minigene cassette. No mRNA was detected in hemizygous male mice as assayed by RT-PCR analysis. |
Mutations Made By | Darrel Stafford, University of North Carolina |
This strain originated on a B6;129P2 background and has been backcrossed to C57BL/6 mice for 10 generations. Matings of homozygous females and hemizygous males may be used when maintaining a live colony.
When using the B6.129P2-F9tm1Dws/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004303 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X-linked: Heterozygous females and wildtype males for F9<tm1Dws> |
Frozen Mouse Embryo | B6.129P2-F9<tm1Dws>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-F9<tm1Dws>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-F9<tm1Dws>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-F9<tm1Dws>/J Frozen Embryos | $3373.50 |
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