Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from cryorecovery.
Mutant mice display impaired fungicidal activity due to myeloperoxidase deficiency. Under hyperlipidemic conditions, mutant mice develop larger atherosclerotic lesions than control mice. These mice are useful for studying systemic inflammatory defenses against pathogens.Aldons J. Lusis, University of California at Los Angeles
Genetic Background | Generation |
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N10+N4F9
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Mpo | myeloperoxidase |
Starting at:
$236.78 Domestic price for female 4-week |
$2,854.50 Domestic price Cryo Recovery |
Mice that are homozygous null for the targeted gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Mpo gene product (mRNA or protein) is detected. Mutant mice exhibit total white blood cell counts and differentials similar to wildtype mice. Neutrophils and monocytes in periperhal blood and bone marrow have no endogenous peroxidase activity. Superoxide production levels in peritoneal exudate cells of mutant mice are similar to levels found in wildtype mice. Hypochlorous acid production is undetectable in both isolated leukocytes and peritoneal exudate cells. Mutant mice display impaired fungicidal activity due to myeloperoxidase deficiency. When maintained under hyperlipidemic conditions, mutant mice develop atherosclerotic lesions 50% larger than those seen in control mice.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the targeted gene encoding exon 7. The targeting vector was constructed to insert a premature stop codon at the end of the light chain of the myeloperoxidase enzyme. The construct was electroporated into 129X1/SvJ derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice using a marker-assisted protocol.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 15, was segregating with 129.
Allele Name | targeted mutation 1, Aldons J Lusis |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Mpo, myeloperoxidase |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 11 |
Molecular Note | Exon 7, which includes sequence involved in heme binding, was disrupted by the insertion of a neomycin selection cassette and a premature stop codon, which precludes the translation of sequence encoding the heavy chain. Northern and Western blot analyses of bone marrow confirmed the absence of transcript and protein in homozygous mutant mice. |
Mutations Made By | Aldons Lusis, University of California at Los Angeles |
This strain originated on a 129X1/SvJ background and has been backcrossed to C57BL/6J for at least ten generations(12/17/01).
When using the MPO KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004265 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Homozygous for Mpo<tm1Lus>, 1 pair minimum |
Frozen Mouse Embryo | B6.129X1-Mpo<tm1Lus>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Mpo<tm1Lus>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Mpo<tm1Lus>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Mpo<tm1Lus>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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