NOD.Cg-Emv30^b Prkdc^scid Tg(TcrLCMV)327Sdz/DvsJ

Stock number: 004257
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These mice do not develop diabetes; The presence of the T cell receptor expressing transgene does not alter the resistance to type 1 diabetes conferred to the NOD background by homozygosity for the scid mutation.

Detailed description

These mice do not develop diabetes; The presence of the T cell receptor expressing transgene does not alter the resistance to type 1 diabetes conferred to the NOD background by homozygosity for the scid mutation. Because this strain is homozygous for the Emv30^b allele, which is the absence of this proviral insertion, these mice have a much lower incidence of thymic lymphoma progression than normally found in mice on the NOD background.

Development

This strain was generated by repeated backcrossing of Tg(TcrLCMV)327Sdz from a segregating B6;D2 background onto NOD.Cg-Prkdc^scid Emv30^b/Dvs. At N9 mice were sibling intercrossed to reach homozygosity for Prkdc^scid and Emv30^b and this strain was maintained segregating for Tg(TcrLCMV)327Sdz. Embryos were generated for cryopreserved from Tg/? Males and females at generation N9F24 so cryorecovered pups should be homozygous for Prkdc^scid and Emv30^b and segregating for the transgene. (Serreze DV et al 2001 Diabetes 50(9):1992)

Allele

Symbol: Tg(TcrLCMV)327Sdz
Name: transgene insertion 327, Birgit Ledermann
MGI accession ID: MGI:2665105
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TcrLCMV)327Sdz
Marker name: transgene insertion 327, Birgit Ledermann
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: Tcrb,T cell receptor beta chain,mouse, laboratory; Tcra,T cell receptor alpha chain,mouse, laboratory
Molecular note: The transgene carries both Tcra and Tcrb. Ten to twenty copies of the Tcr transgene were reported to have integrated on the same chromosome. At least two transgenic lines are known to exist. Line 327 is the representative line. The second line is 318.
General note: Transgenic mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for the gp33 protein of LCMV (lymphocytic choriomeningitis virus), H2-D^b.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Emv30^b
Name: endogenous ecotropic murine leukemia virus, absent
MGI accession ID: MGI:1857806
Generation method: Not Applicable
Marker symbol: Emv30
Marker name: endogenous ecotropic MuLV 30
Chromosome: 11
Strain of origin: Not Applicable
General note: The provirus is missing from C57BLKS/J and from the NOR/LtDn stock which was derived from an NOD subline genetically contaminated with C57BLKS/J (J:14300, J:29951).