NOD.B6-Tg(ML5sHEL)5Ccg/Dvs

Stock number: 004252

Development

Tg(ML5sHEL)5Ccg was backcrossed from C57BL/6-Tg(ML5sHEL)5Ccg (Stock#002599) onto NOD/Lt to generate NOD.B6-Tg(ML5sHEL)5Ccg/Dvs. It has been observed that agouti coat color co-segregates with this transgene even at N11. Thus, albino mice likely are not carriers while agouti mice likely are.

Allele

Symbol: Tg(ML5sHEL)5Ccg
Name: transgene insertion 5, Christopher C Goodnow
MGI accession ID: MGI:2384160
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: MD5; ML5; ML-5; sHEL; solHEL; SWHEL
Marker symbol: Tg(ML5sHEL)5Ccg
Marker name: transgene insertion 5, Christopher C Goodnow
Marker synonyms: MD5; ML5; ML-5; sHEL; solHEL
Chromosome: UN
Strain of origin: C57BL/6JSfd
Expressed genes: HEL,HEL,chicken
Molecular note: A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to a mouse metallothionein I promoter. The mouse metallothionein I promoter is active during embryonic, fetal, and adult life, and can be induced to higher levels of transcription by heavy metals. Mice carrying the transgene had measurable, soluble chicken lysozyme in their serum. The line chosen for study (ML-5) had the highest serum lysozyme concentrations.