Overview

This strain has been cryopreserved as Stock No. 004352.

Genetic overview

Genetic Background	Generation

Ifngr2^tm1Pbro

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ifngr2	interferon gamma receptor 2

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Research Applications

Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research

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Details

Detailed Description

The Ifngr2 allele was made in W9.5 ES cells (129S1/Sv-p⁺ Tyr⁺ Kitl^Sl-J/+ background) and was imported into The Jackson Laboratory from Paul Rothman on a B6,129 segregating background. It was backcrossed onto NOD/Lt and a genome scan was run to select for NOD/Lt markers at Idd1 through Idd15 (performed as described in Serreze et al., J Exp Med, 1996). This strain was then fixed homozygous at N8. It is maintained by sibling breeding homozygotes. (Serreze et al., 2000, Lu et al., 1998.)

Selected References

Serreze DV; Post CM; Chapman HD; Johnson EA; Lu B; Rothman PB. 2000. Interferon-gamma receptor signaling is dispensable in the development of autoimmune type 1 diabetes in NOD mice. Diabetes 49(12):2007-11PubMed: 11118001 MGI: J:65986

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Genetics

Ifngr2^tm1Pbro

Allele Symbol: Ifngr2^tm1Pbro

Allele Name	targeted mutation 1, Paul B Rothman
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	IFN-gammaR2 -; IFNgammaRB^null; Ifngr2^tm1Cmb
Gene Symbol and Name	Ifngr2, interferon gamma receptor 2
Gene Synonym(s)
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	16
Molecular Note	Replacement of the second coding exon with a neomycin gene.
Mutations Made By	Paul Rothman, Johns Hopkins University

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

type 1 diabetes mellitus

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ifngr2^tm1Pbro related

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM)
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
- Inflammation

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Ifngr2^tm1Pbro/Ifngr2^tm1Pbro
involves: 129S1/Sv

hematopoietic system phenotype

abnormal T-helper 1 cell differentiation
- differentiation of Th1 cells inhibited

abnormal class switch recombination
- class switching to IgE and IgG1 caused by Il-4 fails
- class switching to IgG2a inhibited when stimulated with IFN-gamma

immune system phenotype

increased susceptibility to bacterial infection
- increased susceptibility to Listeria monocytogenes infection

decreased susceptibility to type IV hypersensitivity reaction

abnormal T-helper 1 cell differentiation
- differentiation of Th1 cells inhibited

abnormal class switch recombination
- class switching to IgE and IgG1 caused by Il-4 fails
- class switching to IgG2a inhibited when stimulated with IFN-gamma

The following phenotype relates to a compound genotype created using this strain

Genotype: Ifngr2^tm1Pbro/Ifngr2^tm1Pbro
NOD.129S1-Ifngr2

cellular phenotype

abnormal diapedesis
- CD8+ T cells injected into Ifngr2-deficient NOD mice are less able to cause diabetes, due to impaired homing ability and impaired diapedesis

hematopoietic system phenotype

abnormal cytotoxic T cell physiology
- CD8+ T cells injected into Ifngr2-deficient NOD mice are less able to cause diabetes, due to impaired homing ability and impaired diapedesis

abnormal T-helper 2 physiology
- enhanced Th2 responses

abnormal diapedesis
- CD8+ T cells injected into Ifngr2-deficient NOD mice are less able to cause diabetes, due to impaired homing ability and impaired diapedesis

immune system phenotype

abnormal diapedesis
- CD8+ T cells injected into Ifngr2-deficient NOD mice are less able to cause diabetes, due to impaired homing ability and impaired diapedesis

decreased susceptibility to autoimmune diabetes
- when diabetogenic splenocytes are injected into Ifngr2-deficient NOD mice, diabetes development is delayed compared to wild-type NOD mice

abnormal T-helper 2 physiology
- enhanced Th2 responses

abnormal cytotoxic T cell physiology
- CD8+ T cells injected into Ifngr2-deficient NOD mice are less able to cause diabetes, due to impaired homing ability and impaired diapedesis

mammalian phenotype

homeostasis/metabolism phenotype
- Normal - unexpectedly, incidence of type 1 diabetes in females was unchanged

References

Serreze DV; Post CM; Chapman HD; Johnson EA; Lu B; Rothman PB. 2000. Interferon-gamma receptor signaling is dispensable in the development of autoimmune type 1 diabetes in NOD mice. Diabetes 49(12):2007-11PubMed: 11118001 MGI: J:65986

Additional References

Lu B; Ebensperger C; Dembic Z; Wang Y; Kvatyuk M; Lu T; Coffman RL; Pestka S; Rothman PB. 1998. Targeted disruption of the interferon-gamma receptor 2 gene results in severe immune defects in mice. Proc Natl Acad Sci U S A 95(14):8233-8PubMed: 9653170 MGI: J:48016
Serreze DV; Chapman HD; Varnum DS; Hanson MS; Reifsnyder PC; Richard SD; Fleming SA; Leiter EH; Shultz LD. 1996. B lymphocytes are essential for the initiation of T cell-mediated autoimmune diabetes: analysis of a new speed congenic stock of NOD.Ig mu null mice. J Exp Med 184(5):2049-53PubMed: 8920894 MGI: J:37287

Additional - Ifngr2^tm1Pbro related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino, pink eyed
  Related Genotype: A/A Tyr^c/Tyr^c

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Licensing Information

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Genetic overview

Research Applications

Detailed Description

Selected References

Ifngr2tm1Pbro

Allele Symbol: Ifngr2tm1Pbro

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ifngr2tm1Pbro related

Mammalian Phenotype Terms by Genotype

Genotype: Ifngr2tm1Pbro/Ifngr2tm1Pbroinvolves: 129S1/Sv

hematopoietic system phenotype

immune system phenotype

Genotype: Ifngr2tm1Pbro/Ifngr2tm1PbroNOD.129S1-Ifngr2

cellular phenotype

hematopoietic system phenotype

immune system phenotype

mammalian phenotype

References

Additional References

Additional - Ifngr2tm1Pbro related

Genotyping Protocols

Appearance

Terms Of Use

Licensing Information

Ifngr2^tm1Pbro

Allele Symbol: Ifngr2^tm1Pbro

Genotype: Ifngr2^tm1Pbro related

Genotype: Ifngr2^tm1Pbro/Ifngr2^tm1Pbro
involves: 129S1/Sv

Genotype: Ifngr2^tm1Pbro/Ifngr2^tm1Pbro
NOD.129S1-Ifngr2

Additional - Ifngr2^tm1Pbro related