Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No targeted allele product (mRNA or protein) is detected by Northern blot or immunoassay. Mutant mice exhibit mild neutrophilia. Impaired early neutrophil migration in thioglycolate-induced peritonitis is followed by a delayed recovery to nearly normal levels. Although early trauma-induced leukocyte adhesion and migration is greatly reduced and in vivo leukocyte rolling (leukocyte-endothelial cell interaction) in postcapillary venules is severely decreased, cytokine-induced/E selectin-mediated leukocyte rolling is only slightly reduced in the mutant mice. This mutant mouse strain represents a model that may be useful in studies of leukocyte adhesion and migration in the inflammatory response.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a 5' portion of the Selpl coding sequence. The construct was electroporated into STOCK(129/Sv(75%),C57BL/6J(20%),SJL(5%)) derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6J mice. Heterozygotes were bred to generate homozygotes.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (2 of 5 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1, Bruce Furie|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Selplg, selectin, platelet (p-selectin) ligand|
|Strain of Origin||STOCK 129/Sv and C57BL/6J and SJL|
|Molecular Note||A neomycin resistance cassette replaced a small DNA segment of the coding region (+69 to 94). A 300 bp segment in the 5' flanking region containing CT repeats was also deleted. Northern blot analysis on thymus and spleen RNA from homozygous mice demonstrated that no detecable transcript was produced from this allele. Flow cytometry experiments on leukocytes derived from homozygous mice confirmed that no detectable cell surface protein was expressed from this allele.|
|Mutations Made By|| |
Bruce Furie, Beth Israel Deaconess Med Cntr (Harvard)
This strain originated on a STOCK background, has been backcrossed for 5 generations on the C57BL/6J background before being made homozygous. Coat color expected from breeding:Black
When using the PSGL-1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004201 in your Materials and Methods section.