These OT-II transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 co-receptor and is specific for chicken ovalbumin 323-339 peptide in the context of I-Ab (CD4 co-receptor interaction with MHC class II). This results in CD4+ T cells that primarily recognize ovalbumin peptide residues 323-339 when presented by the MHC class II molecule. In these mice there is a four-fold increase in the CD4+ to CD8+ peripheral T cell ratio, and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin peptide (OVA323-339). Immune response dynamics can be studied by direct administration of ovalbumin. These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, and central and peripheral T cell tolerance and induction. Like most TCR transgenics, these mice are somewhat immunodeficient.
Frank Carbone, University of Melbourne
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These OT-II transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T cell ratio, and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, process of thymic selection and central and peripheral T cell tolerance and induction.
Transgenic constructs containing the alpha-chain and beta-chain of the T-cell receptor from a T cell hybridoma clone including the endogenous TCR promoter were coinjected into fertilized B6 x B6.C-H2bm1 mouse eggs. The T cell hybridoma clone is CD4+, MHC class II-restricted and is specific for the ovalbumin residue 323-339 peptides in the context of the MHC class II I-A2 molecule. Founder animals (described as OT-II.2 subline 425-2) were bred to wild-type B6 mice. The strain was crossed to wild-type C57BL/6 for four generations before being made homozygous. The Donating Investigator confirmed the H2bm1 was bred out of the strain.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 425, Frank Carbone |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | OT2; OT-2; OTII; OT-II; OT-II alphabeta TCR; OTII TCR; OT-II.2; TCR-OT-II.2a; TCRTg; Tg(TcraTcrb)425-2Cbn; TgN(OT-II.2a); TgN(TcrOT-II) |
Gene Symbol and Name | Tg(TcraTcrb)425Cbn, transgene insertion 425, Frank Carbone |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | C57BL/6 x B6.C-H2bm1 |
Chromosome | UN |
General Note | Transgenic mice express the mouse alpha-chain and beta-chain T-cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous transgenic mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T-cell ratio, and lymph node T-cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. Phenotypes for the two sublines (OT-II.2)425-2 and 426-6 are described as identical. (J:87876) |
Molecular Note | The transgene was generated by co-injection of two constructs created by separate cloning into a T-cell receptor expression vector the PCR-amplified cDNAs encoding mouse alpha-chain and beta-chain T-cell receptor subunits expressed by a CD4+ T-cell hybridoma designated Clone 1.1. The reconstituted receptor pairs with the CD4 coreceptor and is specific for chicken ovalbumin residues 323-339 in the context of H2-Ab1 (I-Ab). |
Mutations Made By | Eric Butz, Immunex Corp. |
Coat color expected from breeding is Black.
When using the OT-II mouse strain in a publication, please cite the originating article(s) and include JAX stock #004194 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous for Tg(TcraTcrb)425Cbn |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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