| Allele Name | transgene insertion 425, Frank Carbone |
| Allele Type | Transgenic (Inserted expressed sequence) |
| Allele Synonym(s) | OT2; OT-2; OTII; OT-II; OT-II alphabeta TCR; OTII TCR; OT-II.2; TCR-OT-II.2a; TCRTg; Tg(TcraTcrb)425-2Cbn; TgN(OT-II.2a); TgN(TcrOT-II) |
| Gene Symbol and Name | Tg(TcraTcrb)425Cbn, transgene insertion 425, Frank Carbone |
| Gene Synonym(s) | |
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
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| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
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| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
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| Strain of Origin | C57BL/6 x B6.C-H2bm1 |
| Chromosome | UN |
| General Note | Transgenic mice express the mouse alpha-chain and beta-chain T-cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous transgenic mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T-cell ratio, and lymph node T-cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. Phenotypes for the two sublines (OT-II.2)425-2 and 426-6 are described as identical. (J:87876) |
| Molecular Note | The transgene was generated by co-injection of two constructs created by separate cloning into a T-cell receptor expression vector the PCR-amplified cDNAs encoding mouse alpha-chain and beta-chain T-cell receptor subunits expressed by a CD4+ T-cell hybridoma designated Clone 1.1. The reconstituted receptor pairs with the CD4 coreceptor and is specific for chicken ovalbumin residues 323-339 in the context of H2-Ab1 (I-Ab). |
| Mutations Made By | Eric Butz, Immunex Corp. |
