JAX
Mouse Strain Datasheet - 004190
C.129-Il4tm1Lky/J
Also Known As: IL-4/GFP-enhanced transcript (4Get)
The "IL-4/GFP-enhanced transcript" (4Get) allele has a bicistronic IRES-EGFP reporter cassette inserted between the translational stop codon and the 3' UTR of the interleukin 4 gene (Il4); thus expressing the endogenous gene and EGFP. These 4Get mice have fluorescent reporter expression in IL-4-expressing cells, and may be useful for in vivo tracking of both innate and adaptive immune cells, and/or enabling their isolation without further stimulation.
Donating Investigator
Richard M. Locksley, Univ of California San Francisco-HHMI
Genetic overview
| Genetic Background | Generation |
|---|---|
|
N5F?+N3F2
|
Il4tm1Lky
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Reporter) | Il4 | interleukin 4 |
Research Applications
- Immunology, Inflammation and Autoimmunity Research
- Research Tools
- Cancer Research
Detailed Description
Mice homozygous for the "IL-4/GFP-enhanced transcript" (4Get) targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A knockin replaces the endogenous gene with an Il4/IRES/EGFP gene (IL4 activity remains intact). This leads to generation of a bicistronic transcript under the control of endogenous regulatory elements. Cells activated to express IL4 will express EGFP, allowing reliable in vivo tracking of both innate and adaptive immune cells, and/or enabling their isolation without further stimulation. As the IRES element promotes translational competence capable of revealing low-level transcription otherwise not apparent from the canonical 5'-cap of the mRNA, cells from these mice can also be used to report competence for IL4 production upon stimulation.
Development
A targeting vector was designed with a loxP-flanked neomycin resistance cassette, an internal ribosome entry site (IRES), a green fluorescent protein gene, and bovine growth hormone polyadenylation signal. The targeting vector was inserted between the endogenous translational stop codon and the 3' UTR in exon 4 of the interleukin 4 gene (Il4) via electroporation into 129S4/SvJae-derived PrmCre embryonic stem (ES) cells. These PrmCre ES cells contain the Prm-cre transgene with the protamine promoter directing cre expression to the male germline. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred to BALB/cAnNCrlBR females. The male offspring from this initial breeding lack the floxed neo cassette in their germline, and were bred to female BALB/cAnNCrlBR mice. Offspring with the IRES-EGFP reporter cassette and without the Pmc-Cre transgene were identified to establish the colony of "IL-4/GFP-enhanced transcript" (4Get) mice. These 4Get mice were subsequently backcrossed to BALB/cAnNCrlBR mice for at least five generations prior to sending to The Jackson Laboratory Repository in 2002. Upon arrival, the colony was maintained by breeding mutant mice together. In 2007, the colony was bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation, and thereafter maintained by breeding mutant mice together.
In 2009, a 27 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, was performed on the living colony at The Jackson Laboratory Repository. All 27 markers throughout the genome are BALB/c allele type. One marker distinguishing BALB/cJ from BALB/cAn (chromosome 7; 3.8 Mbp) was found to be segregating. Additional SNP analyses in 2011, 2012, and 2014 showed the same result.
Expression Data
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|---|
| Site of Expression | Cells activated to express IL-4 will express GFP. IL-4 activity remains intact. |
Control Suggestions
Selected References
- Mohrs M; Shinkai K; Mohrs K; Locksley RM. 2001. Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter. Immunity 15(2):303-11. PubMed: 11520464MGI: J:75415
Il4tm1Lky
Allele Symbol: Il4tm1Lky
| Allele Name | targeted mutation 1, Richard M Locksley |
|---|---|
| Allele Type | Targeted (Reporter) |
| Allele Synonym(s) | 4 get; 4-get; 4get |
| Gene Symbol and Name | Il4, interleukin 4 |
| Gene Synonym(s) | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Il-4; Il-4; Il4e12 |
| Expressed Gene | GFP, Green Fluorescent Protein, |
| Site of Expression | Cells activated to express IL-4 will express GFP. IL-4 activity remains intact. |
| Strain of Origin | Not Specified |
| Chromosome | 11 |
| Molecular Note | A loxP-flanked neomycin cassette followed by an internal ribosomal entry site (IRES) and an EGFP gene was inserted into sequences corresponding to the 3' untranslated region in exon 4. The neomycin cassette was removed by Cre mediated germline recombination in chimeric mice to generate the final allele. These mice express green fluorescent protein under the control of the Il4 promoter. |
| Mutations Made By | Markus Mohrs, Trudeau Institute, Inc. |
Disease Terms
Research Areas By Genotype
This mouse can be used to support research in many areas including:
- Immunology, Inflammation and Autoimmunity Research
- Intracellular Signaling Molecules
- Research Tools
- Fluorescent Proteins
- Immunology, Inflammation and Autoimmunity Research
Genotype: GFP related
- Research Tools
- Fluorescent Proteins
Genotype: Il4tm1Lky related
- Cancer Research
- Growth Factors/Receptors/Cytokines
- Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
References
- Mohrs M; Shinkai K; Mohrs K; Locksley RM. 2001. Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter. Immunity 15(2):303-11. PubMed: 11520464MGI: J:75415
Additional - Il4tm1Lky related
- Au-Yeung BB; Fowell DJ. 2007. A key role for Itk in both IFN gamma and IL-4 production by NKT cells. J Immunol 179(1):111-9. PubMed: 17579028MGI: J:143156
- Au-Yeung BB; Katzman SD; Fowell DJ. 2006. Cutting edge: Itk-dependent signals required for CD4+ T cells to exert, but not gain, Th2 effector function. J Immunol 176(7):3895-9. PubMed: 16547221MGI: J:129883
- Cain JA; Smith JA; Ondr JK; Wang B; Katz JD. 2006. NKT cells and IFN-gamma establish the regulatory environment for the control of diabetogenic T cells in the nonobese diabetic mouse. J Immunol 176(3):1645-54. PubMed: 16424194MGI: J:126603
- Chen CY; Lee JB; Liu B; Ohta S; Wang PY; Kartashov AV; Mugge L; Abonia JP; Barski A; Izuhara K; Rothenberg ME; Finkelman FD; Hogan SP; Wang YH. 2015. Induction of Interleukin-9-Producing Mucosal Mast Cells Promotes Susceptibility to IgE-Mediated Experimental Food Allergy. Immunity 43(4):788-802. PubMed: 26410628MGI: J:233776
- Cheng LE; Hartmann K; Roers A; Krummel MF; Locksley RM. 2013. Perivascular mast cells dynamically probe cutaneous blood vessels to capture immunoglobulin e. Immunity 38(1):166-75. PubMed: 23290520MGI: J:193023
- Cheng LE; Sullivan BM; Retana LE; Allen CD; Liang HE; Locksley RM. 2015. IgE-activated basophils regulate eosinophil tissue entry by modulating endothelial function. J Exp Med 212(4):513-24. PubMed: 25779634MGI: J:222287
- Cheng LE; Wang ZE; Locksley RM. 2010. Murine B cells regulate serum IgE levels in a CD23-dependent manner. J Immunol 185(9):5040-7. PubMed: 20870945MGI: J:165198
- Clay BS; Shilling RA; Bandukwala HS; Moore TV; Cannon JL; Welcher AA; Weinstock JV; Sperling AI. 2009. Inducible costimulator expression regulates the magnitude of Th2-mediated airway inflammation by regulating the number of Th2 cells. PLoS One 4(11):e7525. PubMed: 19888475MGI: J:155428
- Diny NL; Baldeviano GC; Talor MV; Barin JG; Ong S; Bedja D; Hays AG; Gilotra NA; Coppens I; Rose NR; Cihakova D. 2017. Eosinophil-derived IL-4 drives progression of myocarditis to inflammatory dilated cardiomyopathy. J Exp Med 214(4):943-957. PubMed: 28302646MGI: J:241865
- Dolgachev V; Petersen BC; Budelsky AL; Berlin AA; Lukacs NW. 2009. Pulmonary IL-17E (IL-25) production and IL-17RB+ myeloid cell-derived Th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease. J Immunol 183(9):5705-15. PubMed: 19828636MGI: J:156804
- Everts B; Hussaarts L; Driessen NN; Meevissen MH; Schramm G; van der Ham AJ; van der Hoeven B; Scholzen T; Burgdorf S; Mohrs M; Pearce EJ; Hokke CH; Haas H; Smits HH; Yazdanbakhsh M. 2012. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor. J Exp Med 209(10):1753-67, S1. PubMed: 22966004MGI: J:191421
- Everts B; Perona-Wright G; Smits HH; Hokke CH; van der Ham AJ; Fitzsimmons CM; Doenhoff MJ; van der Bosch J; Mohrs K; Haas H; Mohrs M; Yazdanbakhsh M; Schramm G. 2009. Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses. J Exp Med 206(8):1673-80. PubMed: 19635864MGI: J:151482
- Fairfax KC; Amiel E; King IL; Freitas TC; Mohrs M; Pearce EJ. 2012. IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease. PLoS Pathog 8(1):e1002490. PubMed: 22291593MGI: J:195404
- Fallon PG; Ballantyne SJ; Mangan NE; Barlow JL; Dasvarma A; Hewett DR; McIlgorm A; Jolin HE; McKenzie AN. 2006. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J Exp Med 203(4):1105-16. PubMed: 16606668MGI: J:123748
- Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. PubMed: 19433445MGI: J:150146
- Gagliani N; Magnani CF; Huber S; Gianolini ME; Pala M; Licona-Limon P; Guo B; Herbert DR; Bulfone A; Trentini F; Di Serio C; Bacchetta R; Andreani M; Brockmann L; Gregori S; Flavell RA; Roncarolo MG. 2013. Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med 19(6):739-46. PubMed: 23624599MGI: J:198570
- Gao Y; Nish SA; Jiang R; Hou L; Licona-Limon P; Weinstein JS; Zhao H; Medzhitov R. 2013. Control of T helper 2 responses by transcription factor IRF4-dependent dendritic cells. Immunity 39(4):722-32. PubMed: 24076050MGI: J:208202
- Girtsman T; Jaffar Z; Ferrini M; Shaw P; Roberts K. 2010. Natural Foxp3(+) regulatory T cells inhibit Th2 polarization but are biased toward suppression of Th17-driven lung inflammation. J Leukoc Biol 88(3):537-46. PubMed: 20495073MGI: J:164926
- Goh YP; Henderson NC; Heredia JE; Red Eagle A; Odegaard JI; Lehwald N; Nguyen KD; Sheppard D; Mukundan L; Locksley RM; Chawla A. 2013. Eosinophils secrete IL-4 to facilitate liver regeneration. Proc Natl Acad Sci U S A 110(24):9914-9. PubMed: 23716700MGI: J:197388
- Hadeiba H; Locksley RM. 2003. Lung CD25 CD4 regulatory T cells suppress type 2 immune responses but not bronchial hyperreactivity. J Immunol 170(11):5502-10. PubMed: 12759427MGI: J:109990
- Hammad H; Plantinga M; Deswarte K; Pouliot P; Willart MA; Kool M; Muskens F; Lambrecht BN. 2010. Inflammatory dendritic cells--not basophils--are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen. J Exp Med 207(10):2097-111. PubMed: 20819925MGI: J:165817
- Hams E; McCarron MJ; Amu S; Yagita H; Azuma M; Chen L; Fallon PG. 2011. Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells. J Immunol 186(10):5648-55. PubMed: 21490158MGI: J:173223
- Harris DP; Goodrich S; Mohrs K; Mohrs M; Lund FE. 2005. Cutting edge: the development of IL-4-producing B cells (B effector 2 cells) is controlled by IL-4, IL-4 receptor alpha, and Th2 cells. J Immunol 175(11):7103-7. PubMed: 16301612MGI: J:122200
- Haynes NM; Allen CD; Lesley R; Ansel KM; Killeen N; Cyster JG. 2007. Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation. J Immunol 179(8):5099-108. PubMed: 17911595MGI: J:153037
- Heib V; Becker M; Warger T; Rechtsteiner G; Tertilt C; Klein M; Bopp T; Taube C; Schild H; Schmitt E; Stassen M. 2007. Mast cells are crucial for early inflammation, migration of Langerhans cells, and CTL responses following topical application of TLR7 ligand in mice. Blood 110(3):946-53. PubMed: 17446350MGI: J:145396
- Heredia JE; Mukundan L; Chen FM; Mueller AA; Deo RC; Locksley RM; Rando TA; Chawla A. 2013. Type 2 innate signals stimulate fibro/adipogenic progenitors to facilitate muscle regeneration. Cell 153(2):376-88. PubMed: 23582327MGI: J:214506
- Huber S; Hoffmann R; Muskens F; Voehringer D. 2010. Alternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2. Blood 116(17):3311-20. PubMed: 20625006MGI: J:165869
- Jang S; Schaller M; Berlin AA; Lukacs NW. 2010. Notch Ligand Delta-Like 4 Regulates Development and Pathogenesis of Allergic Airway Responses by Modulating IL-2 Production and Th2 Immunity. J Immunol 185(10):5835-44. PubMed: 20944009MGI: J:165624
- Katzman SD; Fowell DJ. 2008. Pathogen-imposed skewing of mouse chemokine and cytokine expression at the infected tissue site. J Clin Invest 118(2):801-11. PubMed: 18188454MGI: J:131191
- Kim-Saijo M; Janssen EM; Sugie K. 2008. CD4 cell-secreted, posttranslationally modified cytokine GIF suppresses Th2 responses by inhibiting the initiation of IL-4 production. Proc Natl Acad Sci U S A 105(49):19402-7. PubMed: 19036925MGI: J:142099
- King IL; Amiel E; Tighe M; Mohrs K; Veerapen N; Besra G; Mohrs M; Leadbetter EA. 2013. The mechanism of splenic invariant NKT cell activation dictates localization in vivo. J Immunol 191(2):572-82. PubMed: 23785119MGI: J:205445
- King IL; Mohrs M. 2009. IL-4-producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells. J Exp Med 206(5):1001-7. PubMed: 19380638MGI: J:148500
- King SB; Knorn AM; Ohnmacht C; Voehringer D. 2008. Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6. J Immunol 180(2):754-63. PubMed: 18178813MGI: J:130967
- Kobayashi T; Iijima K; Checkel JL; Kita H. 2013. IL-1 family cytokines drive Th2 and Th17 cells to innocuous airborne antigens. Am J Respir Cell Mol Biol 49(6):989-98. PubMed: 23837489MGI: J:231562
- Lazarski CA; Ford J; Katzman SD; Rosenberg AF; Fowell DJ. 2013. IL-4 attenuates Th1-associated chemokine expression and Th1 trafficking to inflamed tissues and limits pathogen clearance. PLoS One 8(8):e71949. PubMed: 23991011MGI: J:206420
- Lee J; Kim TH; Murray F; Li X; Choi SS; Broide DH; Corr M; Lee J; Webster NJ; Insel PA; Raz E. 2015. Cyclic AMP concentrations in dendritic cells induce and regulate Th2 immunity and allergic asthma. Proc Natl Acad Sci U S A 112(5):1529-34. PubMed: 25605931MGI: J:217659
- Liang HE; Reinhardt RL; Bando JK; Sullivan BM; Ho IC; Locksley RM. 2011. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol 13(1):58-66. PubMed: 22138715MGI: J:178986
- Licona-Limon P; Henao-Mejia J; Temann AU; Gagliani N; Licona-Limon I; Ishigame H; Hao L; Herbert DR; Flavell RA. 2013. Th9 Cells Drive Host Immunity against Gastrointestinal Worm Infection. Immunity 39(4):744-57. PubMed: 24138883MGI: J:208730
- Liu AY; Dwyer DF; Jones TG; Bankova LG; Shen S; Katz HR; Austen KF; Gurish MF. 2013. Mast Cells Recruited to Mesenteric Lymph Nodes during Helminth Infection Remain Hypogranular and Produce IL-4 and IL-6. J Immunol 190(4):1758-66. PubMed: 23319739MGI: J:193314
- Liu X; Yan X; Zhong B; Nurieva RI; Wang A; Wang X; Martin-Orozco N; Wang Y; Chang SH; Esplugues E; Flavell RA; Tian Q; Dong C. 2012. Bcl6 expression specifies the T follicular helper cell program in vivo. J Exp Med 209(10):1841-52, S1-24. PubMed: 22987803MGI: J:191271
- Loke P; Gallagher I; Nair MG; Zang X; Brombacher F; Mohrs M; Allison JP; Allen JE. 2007. Alternative activation is an innate response to injury that requires CD4+ T cells to be sustained during chronic infection. J Immunol 179(6):3926-36. PubMed: 17785830MGI: J:152038
- Loke P; Zang X; Hsuan L; Waitz R; Locksley RM; Allen JE; Allison JP. 2005. Inducible costimulator is required for type 2 antibody isotype switching but not T helper cell type 2 responses in chronic nematode infection. Proc Natl Acad Sci U S A 102(28):9872-7. PubMed: 15994233MGI: J:99858
- Macleod MK; McKee AS; David A; Wang J; Mason R; Kappler JW; Marrack P. 2011. Vaccine adjuvants aluminum and monophosphoryl lipid A provide distinct signals to generate protective cytotoxic memory CD8 T cells. Proc Natl Acad Sci U S A 108(19):7914-9. PubMed: 21518876MGI: J:172191
- McHedlidze T; Waldner M; Zopf S; Walker J; Rankin AL; Schuchmann M; Voehringer D; McKenzie AN; Neurath MF; Pflanz S; Wirtz S. 2013. Interleukin-33-dependent innate lymphoid cells mediate hepatic fibrosis. Immunity 39(2):357-71. PubMed: 23954132MGI: J:208227
- McKee AS; MacLeod M; White J; Crawford F; Kappler JW; Marrack P. 2008. Gr1+IL-4-producing innate cells are induced in response to Th2 stimuli and suppress Th1-dependent antibody responses. Int Immunol 20(5):659-69. PubMed: 18343889MGI: J:134492
- McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. PubMed: 19734227MGI: J:152787
- Mingueneau M; Roncagalli R; Gregoire C; Kissenpfennig A; Miazek A; Archambaud C; Wang Y; Perrin P; Bertosio E; Sansoni A; Richelme S; Locksley RM; Aguado E; Malissen M; Malissen B. 2009. Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. Immunity 31(2):197-208. PubMed: 19682930MGI: J:151840
- Mohrs K; Harris DP; Lund FE; Mohrs M. 2005. Systemic dissemination and persistence of Th2 and type 2 cells in response to infection with a strictly enteric nematode parasite. J Immunol 175(8):5306-13. PubMed: 16210636MGI: J:119109
- Mohrs K; Wakil AE; Killeen N; Locksley RM; Mohrs M. 2005. A two-step process for cytokine production revealed by IL-4 dual-reporter mice. Immunity 23(4):419-29. PubMed: 16226507MGI: J:113279
- Molofsky AB; Nussbaum JC; Liang HE; Van Dyken SJ; Cheng LE; Mohapatra A; Chawla A; Locksley RM. 2013. Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages. J Exp Med 210(3):535-49. PubMed: 23420878MGI: J:219842
- Moore ML; Newcomb DC; Parekh VV; Van Kaer L; Collins RD; Zhou W; Goleniewska K; Chi MH; Mitchell D; Boyce JA; Durbin JE; Sturkie C; Peebles RS Jr. 2009. STAT1 negatively regulates lung basophil IL-4 expression induced by respiratory syncytial virus infection. J Immunol 183(3):2016-26. PubMed: 19587017MGI: J:151702
- Mukherjee S; Rasky AJ; Lundy PA; Kittan NA; Kunkel SL; Maillard IP; Kowalski PE; Kousis PC; Guidos CJ; Lukacs NW. 2014. STAT5-induced lunatic fringe during Th2 development alters delta-like 4-mediated Th2 cytokine production in respiratory syncytial virus-exacerbated airway allergic disease. J Immunol 192(3):996-1003. PubMed: 24367028MGI: J:207313
- Nel HJ; Hams E; Saunders SP; Mangan NE; Smith P; Atzberger A; Flavell RA; Akira S; McKenzie AN; Fallon PG. 2011. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice. J Immunol 186(8):4631-9. PubMed: 21398616MGI: J:172526
- Oboki K; Ohno T; Kajiwara N; Arae K; Morita H; Ishii A; Nambu A; Abe T; Kiyonari H; Matsumoto K; Sudo K; Okumura K; Saito H; Nakae S. 2010. IL-33 is a crucial amplifier of innate rather than acquired immunity. Proc Natl Acad Sci U S A 107(43):18581-6. PubMed: 20937871MGI: J:165513
- Ohnmacht C; Schwartz C; Panzer M; Schiedewitz I; Naumann R; Voehringer D. 2010. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity 33(3):364-74. PubMed: 20817571MGI: J:164432
- Ohnmacht C; Voehringer D. 2009. Basophil effector function and homeostasis during helminth infection. Blood 113(12):2816-25. PubMed: 18941115MGI: J:146344
- Palm NW; Rosenstein RK; Yu S; Schenten DD; Florsheim E; Medzhitov R. 2013. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity. Immunity 39(5):976-85. PubMed: 24210353MGI: J:208996
- Parravicini V; Field AC; Tomlinson PD; Albert Basson M; Zamoyska R. 2008. Itch-/- alphabeta and gammadelta T cells independently contribute to autoimmunity in Itchy mice. Blood 111(8):4273-7282. PubMed: 18256323MGI: J:134355
- Patnode ML; Cheng CW; Chou CC; Singer MS; Elin MS; Uchimura K; Crocker PR; Khoo KH; Rosen SD. 2013. Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue. J Biol Chem 288(37):26533-45. PubMed: 23880769MGI: J:203546
- Perona-Wright G; Mohrs K; Mayer KD; Mohrs M. 2010. Differential regulation of IL-4Ralpha expression by antigen versus cytokine stimulation characterizes Th2 progression in vivo. J Immunol 184(2):615-23. PubMed: 20018622MGI: J:159399
- Perona-Wright G; Mohrs K; Mohrs M. 2010. Sustained signaling by canonical helper T cell cytokines throughout the reactive lymph node. Nat Immunol 11(6):520-6. PubMed: 20418876MGI: J:160618
- Perona-Wright G; Mohrs K; Taylor J; Zaph C; Artis D; Pearce EJ; Mohrs M. 2008. Cutting edge: Helminth infection induces IgE in the absence of mu- or delta-chain expression. J Immunol 181(10):6697-701. PubMed: 18981085MGI: J:140953
- Petersen BC; Budelsky AL; Baptist AP; Schaller MA; Lukacs NW. 2012. Interleukin-25 induces type 2 cytokine production in a steroid-resistant interleukin-17RB+ myeloid population that exacerbates asthmatic pathology. Nat Med 18(5):751-8. PubMed: 22543263MGI: J:185138
- Phythian-Adams AT; Cook PC; Lundie RJ; Jones LH; Smith KA; Barr TA; Hochweller K; Anderton SM; Hammerling GJ; Maizels RM; MacDonald AS. 2010. CD11c depletion severely disrupts Th2 induction and development in vivo. J Exp Med 207(10):2089-96. PubMed: 20819926MGI: J:194809
- Piehler D; Stenzel W; Grahnert A; Held J; Richter L; Kohler G; Richter T; Eschke M; Alber G; Muller U. 2011. Eosinophils Contribute to IL-4 Production and Shape the T-Helper Cytokine Profile and Inflammatory Response in Pulmonary Cryptococcosis. Am J Pathol 179(2):733-44. PubMed: 21699881MGI: J:174409
- Price AE; Liang HE; Sullivan BM; Reinhardt RL; Eisley CJ; Erle DJ; Locksley RM. 2010. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A 107(25):11489-94. PubMed: 20534524MGI: J:161387
- Rangel-Moreno J; Moyron-Quiroz JE; Carragher DM; Kusser K; Hartson L; Moquin A; Randall TD. 2009. Omental milky spots develop in the absence of lymphoid tissue-inducer cells and support B and T cell responses to peritoneal antigens. Immunity 30(5):731-43. PubMed: 19427241MGI: J:149548
- Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. PubMed: 17450126MGI: J:122735
- Reinhardt RL; Hong S; Kang SJ; Wang ZE; Locksley RM. 2006. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177(3):1618-27. PubMed: 16849470MGI: J:112745
- Reinhardt RL; Liang HE; Locksley RM. 2009. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol 10(4):385-93. PubMed: 19252490MGI: J:147784
- Scheu S; Stetson DB; Reinhardt RL; Leber JH; Mohrs M; Locksley RM. 2006. Activation of the integrated stress response during T helper cell differentiation. Nat Immunol 7(6):644-51. PubMed: 16680145MGI: J:112676
- Schwartz C; Turqueti-Neves A; Hartmann S; Yu P; Nimmerjahn F; Voehringer D. 2014. Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion. Proc Natl Acad Sci U S A 111(48):E5169-77. PubMed: 25404305MGI: J:216648
- Seidl A; Panzer M; Voehringer D. 2011. Protective immunity against the gastrointestinal nematode Nippostrongylus brasiliensis requires a broad T-cell receptor repertoire. Immunology 134(2):214-23. PubMed: 21896015MGI: J:179222
- Sofi MH; Qiao Y; Ansel KM; Kubo M; Chang CH. 2011. Induction and Maintenance of IL-4 Expression Are Regulated Differently by the 3' Enhancer in CD4 T Cells. J Immunol 186(5):2792-9. PubMed: 21282512MGI: J:169377
- Sokol CL; Barton GM; Farr AG; Medzhitov R. 2008. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol 9(3):310-8. PubMed: 18300366MGI: J:131552
- Sokol CL; Chu NQ; Yu S; Nish SA; Laufer TM; Medzhitov R. 2009. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat Immunol 10(7):713-20. PubMed: 19465907MGI: J:150141
- Sugawara R; Lee EJ; Jang MS; Jeun EJ; Hong CP; Kim JH; Park A; Yun CH; Hong SW; Kim YM; Seoh JY; Jung Y; Surh CD; Miyasaka M; Yang BG; Jang MH. 2016. Small intestinal eosinophils regulate Th17 cells by producing IL-1 receptor antagonist. J Exp Med 213(4):555-67. PubMed: 26951334MGI: J:233006
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