STOCK Tg(Wap-HRAS)69Lln Chr Y^SJL-Edaradd^cr-3J/J

Stock number: 004184
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Endocrine Deficiency Research, Research Tools

Description

These mice are characterized by the development of multiple tumors of the mammary and salivary glands.

Detailed description

Male mice with this Tg(Wap-HRAS)69Lln transgene on a segregating B6;SJL background develop multiple tumors of the mammary and salivary glands by 1 year of age, and on the FVB/N background they develop multiple mammary tumors by age 6-8 weeks. The tumor phenotype has not been characterized on the B6;FVB segregating background, but some males of the Edaradd^cr-3J bearing stock developed visible mammary gland tumors and became moribund between 4 and 8 months of age (LR Donahue, personal communication). The transgene can be eliminated from the stock by crossing a female homozygous for Edaradd^cr-3J with a wild-type male mouse, which will replace the transgene-bearing Y Chromosome with that of the wild-type strain.

Development

Mice exhibitiing the crinkled 3 Jackson (Edaradd^cr-3J) phenotype were found in 2000 by Harold Coombs in Dr. Wesley Beamer's colony of FVB/N-Tg(WapHRAS)69Lln Chr Y^SJL/J mice at The Jackson Laboratory. Edaradd^cr-3J homozygous males were crossed to C57BL/6J females; these pairs and their F1 progeny were donated to Dr. Leah Rae Donahue in the Mouse Mutant Resource. Homozygous F2 male progeny of these crosses were again crossed to C57BL/6J females, and sperm from N2F2 males was cryopreserved. Mice recovered by in vitro fertilization using the frozen sperm and ova from C57BL/6J females will thus be at backcross generation N3 to C57BL/6J.

The background strain on which Edaradd^cr-3J arose was then at backcross generation N14 to FVB/N. It carried, and still carries, a transgene comprising the human Harvey rat sarcoma (HRAS) oncogene homolog under control of the whey acidic protein promoter. The transgene was originally injected into a B6SJLF1 zygote, and it integrated into the Y Chromosome. It was transferred onto and maintained on the FVB/N background by crossing male mice hemizygous for the transgene with wild-type FVB/N female mice for 14 generations, after which it was maintained by crossing hemizygous males with their sisters.

Allele

Symbol: Edaradd^cr-3J
Name: crinkled 3 Jackson
MGI accession ID: MGI:3027289
Generation method: Spontaneous
Marker symbol: Edaradd
Marker name: EDAR (ectodysplasin-A receptor)-associated death domain
Marker synonyms: 1810032E07Rik; 5830469M23Rik; CR; ECTD11A; ECTD11B; ED3; EDA3; EDAR (ectodysplasin-A receptor)-associated death domain; RGD1564010
Chromosome: 13
Strain of origin: FVB.Cg-Tg(Wap-HRAS)69Lln Chr Y^SJL
Molecular note: This spontaneous mutant was identified as an remutation at the Edaradd locus through complementation analysis with B6C3Fe-a/a-Edaradd^cr.
General note:

Extensive characterization has been done for the original crinkled mutation (cr), but comparable data are unavailable for this remutation.

Allele

Symbol: Tg(Wap-HRAS)69Lln
Name: transgene insertion 69, Loretta L Nielsen
MGI accession ID: MGI:3039648
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: HRAS; Tg(WapHRAS)69Lin; Tg(Wap-HRAS)69Lin
Marker symbol: Tg(Wap-HRAS)69Lln
Marker name: transgene insertion 69, Loretta L Nielsen
Marker synonyms: Tg(WapHRAS)69Lin; Tg(Wap-HRAS)69Lin
Chromosome: Y
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: HRAS,HRas proto-oncogene, GTPase,human
Molecular note: This transgene contains the murine whey acidic protein promoter and an allele of the human Harvey rat sarcoma viral oncogene gene homolog that contains a point mutation in codon 12. The transgene integrated into the Y chromosome (SJL).

Allele

MGI accession ID: MGI:3039660
Marker symbol: Tg(Wap-HRAS)69Lln
Marker name: transgene insertion 69, Loretta L Nielsen
Marker synonyms: Tg(WapHRAS)69Lin; Tg(Wap-HRAS)69Lin
Chromosome: Y