|Allele Type||Gene Symbol||Gene Name|
|Spontaneous||Edaradd||EDAR (ectodysplasin-A receptor)-associated death domain|
|Transgenic (Humanized sequence, Inserted expressed sequence)|
|Marker Symbol||Marker Name|
|Tg(Wap-HRAS)69Lln||transgene insertion 69, Loretta L Nielsen|
Male mice with this transgene on a segregating B6;SJL background develop multiple tumors of the mammary and salivary glands by 1 year of age, and on the FVB/N background they develop multiple mammary tumors by age 6-8 weeks; the tumor phenotype has not been characterized on the B6;FVB segregating background, but some males of the Edaraddcr-3J bearing stock developed visible mammary gland tumors and became moribund between 4 and 8 months of age (LR Donahue, personal communication). The transgene can be eliminated from the stock by crossing a female homozygous for Edaraddcr-3J with a wild-type male mouse, which will replace the transgene-bearing Y Chromosome with that of the wild-type strain.
Mice exhibitiing the crinkled 3 Jackson (Edaraddcr-3J) phenotype were found in 2000 by Harold Coombs in Dr. Wesley Beamer's colony of FVB/N-Tg(WapHRAS)69Lln Chr YSJL/J mice at The Jackson Laboratory. Edaraddcr-3J homozygous males were crossed to C57BL/6J females; these pairs and their F1 progeny were donated to Dr. Leah Rae Donahue in the Mouse Mutant Resource. Homozygous F2 male progeny of these crosses were again crossed to C57BL/6J females, and sperm from N2F2 males was cryopreserved. Mice recovered by in vitro fertilization using the frozen sperm and ova from C57BL/6J females will thus be at backcross generation N3 to C57BL/6J.
The background strain on which Edaraddcr-3J arose was then at backcross generation N14 to FVB/N. It carried, and still carries, a transgene comprising the human Harvey rat sarcoma (HRAS) oncogene homolog under control of the whey acidic protein promoter. The transgene was originally injected into a B6SJLF1 zygote, and it integrated into the Y Chromosome. It was transferred onto and maintained on the FVB/N background by crossing male mice hemizygous for the transgene with wild-type FVB/N female mice for 14 generations, after which it was maintained by crossing hemizygous males with their sisters.
|Expressed Gene||HRAS, HRas proto-oncogene, GTPase, human|
|Site of Expression|
|Allele Name||crinkled 3 Jackson|
|Gene Symbol and Name||Edaradd, EDAR (ectodysplasin-A receptor)-associated death domain|
|Gene Synonym(s)||1810032E07Rik; 1810032E07Rik; 5830469M23Rik; 5830469M23Rik; ECTD11A; ECTD11B; ED3; EDA3; RGD1564010; RIKEN cDNA 1810032E07 gene; RIKEN cDNA 5830469M23 gene; cr; crinkled|
|Strain of Origin||FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL|
|General Note|| |
Extensive characterization has been done for the original crinkled mutation (cr), but comparable data are unavailable for this remutation.
|Molecular Note||This spontaneous mutant was identified as an remutation at the Edaradd locus through complementation analysis with B6C3Fe-a/a-Edaraddcr.|
|Allele Name||transgene insertion 69, Loretta L Nielsen|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Allele Synonym(s)||Tg(Wap-HRAS)69Lin; Tg(WapHRAS)69Lin|
|Gene Symbol and Name||Tg(Wap-HRAS)69Lln, transgene insertion 69, Loretta L Nielsen|
|Gene Synonym(s)||Tg(Wap-HRAS)69Lin; Tg(WapHRAS)69Lin|
|Promoter||Wap, whey acidic protein, mouse, laboratory|
|Expressed Gene||HRAS, HRas proto-oncogene, GTPase, human|
|Strain of Origin||(C57BL/6 x SJL)F1|
|Molecular Note||This transgene contains the murine whey acidic protein promoter and an allele of the human Harvey rat sarcoma viral oncogene gene homolog that contains a point mutation in codon 12. The transgene integrated into the Y chromosome (SJL).|
|Mutations Made By|| |
Dr. Loretta Nielsen, Amyllin Pharmaceuticals Inc.
|Marker Synonym(s)||Tg(Wap-HRAS)69Lin; Tg(WapHRAS)69Lin|
|Hemizygous for Edaradd<cr-3J>,Y linked - Tg(Wap-HRAS)69Lln Chr Y<SJL>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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