STOCK Tg(Wap-HRAS)69Lln Chr Y^SJL-Edaradd^cr-3J/J

Stock number: 004184

Description

These mice are characterized by the development of multiple tumors of the mammary and salivary glands.

Detailed description

Male mice with this transgene on a segregating B6;SJL background develop multiple tumors of the mammary and salivary glands by 1 year of age, and on the FVB/N background they develop multiple mammary tumors by age 6-8 weeks; the tumor phenotype has not been characterized on the B6;FVB segregating background, but some males of the Edaradd^cr-3J bearing stock developed visible mammary gland tumors and became moribund between 4 and 8 months of age (LR Donahue, personal communication). The transgene can be eliminated from the stock by crossing a female homozygous for Edaradd^cr-3J with a wild-type male mouse, which will replace the transgene-bearing Y Chromosome with that of the wild-type strain.

Development

Mice exhibitiing the crinkled 3 Jackson (Edaradd^cr-3J) phenotype were found in 2000 by Harold Coombs in Dr. Wesley Beamer's colony of FVB/N-Tg(WapHRAS)69Lln Chr Y^SJL/J mice at The Jackson Laboratory. Edaradd^cr-3J homozygous males were crossed to C57BL/6J females; these pairs and their F1 progeny were donated to Dr. Leah Rae Donahue in the Mouse Mutant Resource. Homozygous F2 male progeny of these crosses were again crossed to C57BL/6J females, and sperm from N2F2 males was cryopreserved. Mice recovered by in vitro fertilization using the frozen sperm and ova from C57BL/6J females will thus be at backcross generation N3 to C57BL/6J.

The background strain on which Edaradd^cr-3J arose was then at backcross generation N14 to FVB/N. It carried, and still carries, a transgene comprising the human Harvey rat sarcoma (HRAS) oncogene homolog under control of the whey acidic protein promoter. The transgene was originally injected into a B6SJLF1 zygote, and it integrated into the Y Chromosome. It was transferred onto and maintained on the FVB/N background by crossing male mice hemizygous for the transgene with wild-type FVB/N female mice for 14 generations, after which it was maintained by crossing hemizygous males with their sisters.

Allele

Symbol: Edaradd^cr-3J
Name: crinkled 3 Jackson
MGI accession ID: MGI:3027289
Generation method: Spontaneous
Marker symbol: Edaradd
Marker name: EDAR (ectodysplasin-A receptor)-associated death domain
Chromosome: 13
Strain of origin: FVB.Cg-Tg(Wap-HRAS)69Lln Chr Y^SJL
Molecular note: This spontaneous mutant was identified as an remutation at the Edaradd locus through complementation analysis with B6C3Fe-a/a-Edaradd^cr.
General note:

Extensive characterization has been done for the original crinkled mutation (cr), but comparable data are unavailable for this remutation.

Allele

Symbol: Tg(Wap-HRAS)69Lln
Name: transgene insertion 69, Loretta L Nielsen
MGI accession ID: MGI:3039648
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Wap-HRAS)69Lln
Marker name: transgene insertion 69, Loretta L Nielsen
Chromosome: Y
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: HRAS,HRas proto-oncogene, GTPase,human
Molecular note: This transgene contains the murine whey acidic protein promoter and an allele of the human Harvey rat sarcoma viral oncogene gene homolog that contains a point mutation in codon 12. The transgene integrated into the Y chromosome (SJL).

Allele

MGI accession ID: MGI:3039660
Marker symbol: Tg(Wap-HRAS)69Lln
Marker name: transgene insertion 69, Loretta L Nielsen
Chromosome: Y