On the C57BL/6J background, most mice homozygous for the targeted allele die during postnatal week 1-2 (On a 129 background, most survived to postnatal week 4-6). These older, surviving mice fail to open their eyes. Upon inspection, the eye is found to be smaller than that seen in wildtype mice with the pupil being widely dilated and unresponsive to light. The urinary bladders of these mice are severely distended with urine and in the case of older animals, urinary stones. Nicotine fails to elicit a contractile response in bladder tissue derived from homozygotes. Heterozygotes are phenotypically normal.

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These <i> Chrna3</i> knock-out mice exhibit increased mortality before and after weaning with bladder defects and unresponsive ocular pupils. They may be useful in applications related to the study of megacystis-microcolon-intestinal hypoperistalsis syndrome in humans.

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