These Chrna3 knock-out mice exhibit increased mortality before and after weaning with bladder defects and unresponsive ocular pupils. They may be useful in applications related to the study of megacystis-microcolon-intestinal hypoperistalsis syndrome in humans.
Dr. Arthur Beaudet, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Chrna3 | cholinergic receptor, nicotinic, alpha polypeptide 3 |
On the C57BL/6J background, most mice homozygous for the targeted allele die during postnatal week 1-2 (On a 129 background, most survived to postnatal week 4-6). These older, surviving mice fail to open their eyes. Upon inspection, the eye is found to be smaller than that seen in wildtype mice with the pupil being widely dilated and unresponsive to light. The urinary bladders of these mice are severely distended with urine and in the case of older animals, urinary stones. Nicotine fails to elicit a contractile response in bladder tissue derived from homozygotes. Heterozygotes are phenotypically normal.
A targeting vector containing hypoxanthine phosphoribosyltransferase (HPRT) and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Chrna3 gene encoding exon 5. The construct was electroporated into 129S6/SvEvBrd-Hprtb-m2-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were mated to C57BL/6 females.
Allele Name | targeted mutation 1, Baylor College of Medicine |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alpha3 - |
Gene Symbol and Name | Chrna3, cholinergic receptor, nicotinic, alpha polypeptide 3 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 9 |
Molecular Note | A 1.8kb genomic fragment containing exon 3 was replaced by an HPRT minigene. These sequences encode three of the four transmembrane domains, the amphipathic helix domain and the four cysteines essential for ligand binding. No transcript produced from this allele was detectable by northern blotting. |
Mutations Made By | Dr. Arthur Beaudet, Baylor College of Medicine |
This strain originated on a B6;129S7 background and has been backcrossed to C57BL/6 for ten generations (8/01). It is maintained as a heterozygote.
When using the α3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004164 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Chrna3<tm1Bay> |
Frozen Mouse Embryo | B6.129S7-Chrna3<tm1Bay>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Chrna3<tm1Bay>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Chrna3<tm1Bay>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Chrna3<tm1Bay>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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