These Cdk5r knock-out mice exhibit defects in the patterning of the cerebral cortex and other areas of the brain.
Li-Huei Tsai, Massachusetts Institue of Technology
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cdk5r1 | cyclin-dependent kinase 5, regulatory subunit 1 (p35) |
At birth, mice homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cdk5r protein product is not immunodetectable in homozygote whole-brain lysates. Histological analysis of homozygotes reveals severe defects in the patterning of the cerebral cortex and other areas of the brain. The normal lamination pattern of cortical neurons is disrupted; axonal trajectories and dendritic structures are altered. Homozygous mice are more susceptible to mortality from chemically induced-seizures. Sporadic adult lethality is also observed, presumably resulting from spontaneous seizures, a consequence of the disrupted cytoarchitecture observed in the cortex. This mutant mouse strain represents a model that may be useful in studies related to the mechanisms of cortical lamination, epilepsy, Alzheimer's and other neurodegenerative diseases.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the entire Cdk5r open reading frame and 400 bp of downstream sequence. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to female C57BL/6 mice.
Allele Name | targeted mutation 1, Li-Huei Tsai |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p35- |
Gene Symbol and Name | Cdk5r1, cyclin-dependent kinase 5, regulatory subunit 1 (p35) |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced 1.4 kb of sequence, including the entire open reading frame. |
Mutations Made By | Li-Huei Tsai, Massachusetts Institue of Technology |
This strain originated on a B6;129S4 background and has been backcrossed to C57BL/6 for at least 5 generations.
When using the p35- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004163 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Cdk5r1<tm1Lht> |
Frozen Mouse Embryo | B6.129S4-Cdk5r1<tm1Lht>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cdk5r1<tm1Lht>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cdk5r1<tm1Lht>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Cdk5r1<tm1Lht>/J Frozen Embryos | $3373.50 |
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