These floxed mutant mice possess loxP sites located 3 kb upstream of exon 1 and in intron 1 of the Scap gene and are useful for generating tissue-specific knockout mutants of Scap.
Dr. Jay D. Horton, Univ of Texas SW Med Center at Dallas
Mice that are homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They posses a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. A third loxP site is located in intron 1. When used in conjunction with a Cre recombinase-expressing strain, this strain is appropriate for generating tissue-specific knockout mutants of Scap. This mutant mouse strain represents a model that may be useful in studies related to cholesterol and fatty acid homeostasis.
A targeting vector containing a loxP-flanked neo cassette located 3kb 5' of Scap exon 1 and a third loxP site located in intron 1, was electroporated into 129S6/SvEv-derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.
|Allele Name||targeted mutation 1, Joseph L Goldstein|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Scap-; SCAPflox; Scaptm1Gsn|
|Gene Symbol and Name||Scap, SREBF chaperone|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. An additional loxP site was inserted in intron 1.|
|Mutations Made By|| |
Dr. Jay Horton, Univ of Texas SW Med Center at Dallas
This strain originated on a B6;129S6 background. It is maintained as a homozygote on the same background.
When using the SCAPf mouse strain in a publication, please cite the originating article(s) and include JAX stock #004162 in your Materials and Methods section.