B6;129-Scap^tm1Mbjg/J

Stock number: 004162
Product name: SCAP^f
Research areas: Metabolism Research, Research Tools

Description

These floxed mutant mice possess loxP sites located 3 kb upstream of exon 1 and in intron 1 of the Scap gene and are useful for generating tissue-specific knockout mutants of Scap.

Detailed description

Mice that are homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They posses a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. A third loxP site is located in intron 1. When used in conjunction with a Cre recombinase-expressing strain, this strain is appropriate for generating tissue-specific knockout mutants of Scap. This mutant mouse strain represents a model that may be useful in studies related to cholesterol and fatty acid homeostasis.

Development

A targeting vector containing a loxP-flanked neo cassette located 3kb 5' of Scap exon 1 and a third loxP site located in intron 1, was electroporated into 129S6/SvEv-derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.

Allele

Symbol: Scap^tm1Mbjg
Name: targeted mutation 1, Joseph L Goldstein
MGI accession ID: MGI:2148743
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Scap
Marker name: SREBF chaperone
Chromosome: 9
Strain of origin: 129S6/SvEvTac
Molecular note: A a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. An additional loxP site was inserted in intron 1.