KGF knockout mice are fibroblast growth factor 7 deficient, and may be useful in studying kidney disease as well as spleen hypoplasia, abnormal synaptic vesicle clustering and miniature inhibitory postsynaptic currents, increased susceptibility to drug-induced seizures, and impaired thymic recovery after injury.
Elaine Fuchs, The Rockefeller University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fgf7 | fibroblast growth factor 7 |
Mice homozygous for this targeted allele are viable, fertile and normal in size. No Fgf7 transcript is detected. By two months of age, the hair coat takes on a matted/greasy appearance that becomes more prominent with age. Homozygote kidneys are markedly smaller and possess structural anomalies. Morphometric analyses indicate a reduction (~30% of wildtype) in the total number of nephrons present. Kidney development appears to be effected as early as embryonic day 16.5. This mutant mouse strain represents a model that may be useful in studies related to kidney disease.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.
Allele Name | targeted mutation 1, Elaine Fuchs |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FGF7-; Fgftm1Efu; KGF- |
Gene Symbol and Name | Fgf7, fibroblast growth factor 7 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | Replacement of 205bp in exon 1 with a neomycin resistance cassette. |
Mutations Made By | Linda Degenstein, The University of Chicago |
This strain originated on a B6;129 background. The live colony is maintained on the same background by homozygous matings.
When using the B6;129-Fgf7tm1Efu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004161 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Fgf7<tm1Efu>, 1 pair minimum |
Frozen Mouse Embryo | B6;129-Fgf7tm1Efu/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Fgf7tm1Efu/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Fgf7tm1Efu/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129-Fgf7tm1Efu/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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