KGF knockout mice are fibroblast growth factor 7 deficient, and may be useful in studying kidney disease as well as spleen hypoplasia, abnormal synaptic vesicle clustering and miniature inhibitory postsynaptic currents, increased susceptibility to drug-induced seizures, and impaired thymic recovery after injury.
Elaine Fuchs, The Rockefeller University
Mice homozygous for this targeted allele are viable, fertile and normal in size. No Fgf7 transcript is detected. By two months of age, the hair coat takes on a matted/greasy appearance that becomes more prominent with age. Homozygote kidneys are markedly smaller and possess structural anomalies. Morphometric analyses indicate a reduction (~30% of wildtype) in the total number of nephrons present. Kidney development appears to be effected as early as embryonic day 16.5. This mutant mouse strain represents a model that may be useful in studies related to kidney disease.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.
|Allele Name||targeted mutation 1, Elaine Fuchs|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||FGF7-; Fgftm1Efu; KGF-|
|Gene Symbol and Name||Fgf7, fibroblast growth factor 7|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Replacement of 205bp in exon 1 with a neomycin resistance cassette.|
|Mutations Made By|| |
Linda Degenstein, The University of Chicago
This strain originated on a B6;129 background. The live colony is maintained on the same background by homozygous matings.
When using the B6;129-Fgf7tm1Efu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004161 in your Materials and Methods section.