B6.129-Ctnnb1^tm2Kem/KnwJ

Stock number: 004152
Product name: B-catenin^flox
Research areas: Developmental Biology Research, Research Tools

Description

When bred to different strains expressing Cre recombinase in various tissues, this strain may be useful in studies such as chrondocyte differentiation, cardiovascular disease, brain malformation and studies of craniofacial, lung, bone and tooth development.

Detailed description

These mice possess loxP sites located in introns 1 and 6 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in chrondocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of chrondocyte differentiation.

When bred to a strain expressing Cre recombinase in heart(see Stock No. 005650 or 005657 for example), this mutant mouse strain may be useful in studies of cardiovascular disease.

When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of brain malformation and craniofacial development.

When bred to a strain expressing Cre recombinase in the distal posterior region of the embryo (see Stock No. 005622 for example), this mutant mouse strain may be useful in studies of lung development.

When bred to a strain expressing Cre recombinase in palate (see Stock No. 009388 for example), this mutant mouse strain may be useful in studies of tooth development.

When bred to a strain expressing Cre recombinase in early limb bud mesenchyme and a subset of craniofacial mesenchyme (see Stock No. 005584 for example), this mutant mouse strain may be useful in studies of bone development.

When bred to mice carrying Tg(Krt1-15-cre/PGR)22Cot (Stock No. 005249), RU 486-induced Cre recombinase expression in the epithelial and hair follicle results in altered hair growth.

When bred to mice carrying Tg(KRT14-cre/ERT)20Efu (Stock No. 005107), tamoxifen-inducible Cre recombinase expression in the epidermis results in increased cell proliferation and increased numbers of mast cells.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. Similarly oriented loxP sites were placed upstream of exon 2 and flanking the neomycin resistance/ thymidine kinase genes (located in intron 6). The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 2-6 were injected in C57BL/6J blastocysts. Resulting chimeric male animals were backcrossed to wild-type C57BL/6J mice.

Allele

Symbol: Ctnnb1^tm2Kem
Name: targeted mutation 2, Rolf Kemler
MGI accession ID: MGI:2148567
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ctnnb1
Marker name: catenin (cadherin associated protein), beta 1
Chromosome: 9
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted in intron 1 and a loxP-flanked neomycin-TK cassette was inserted downstream in intron 6. The neomycin-TK cassette was removed by transient transfection with a Cre recombinase expression vector in ES cells prior to the production of chimeric mice and left two loxP sites flanking a region of gene sequence from exon 2 through exon 6.