These Aplp2 knock-out mice exhibit a phenotype similar to wild-type littermates, but show a slight reduction in weight, decreased forelimb grip strength, and decreased locomotor activity than wild-type littermates.
Dr. David R Borchelt, University of Florida
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Aplp2 | amyloid beta (A4) precursor-like protein 2 |
Mice that are homozygous null for the Aplp2 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Aplp2 gene product (mRNA or protein) is detected. This strain may be useful in studies related to Alzheimer's Disease, especially if used in conjunction with other mutant mouse strains (see B6.129S7-Apptm1Dbo, Stock No. 004133).
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Aplp2 gene encoding the promoter and exon 1. The construct was electroporated into 129S7/SvEvBrd-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Allele Name | targeted mutation 1, David R Borchelt |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | APLP2- |
Gene Symbol and Name | Aplp2, amyloid beta (A4) precursor-like protein 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 9 |
Molecular Note | Replacement of the promoter region and first exon with a neomycin cassette. Western blot analysis on samples derived from nervous tissue and other peripheral tissues of homozygous mutant mice failed to detect any protein. |
Mutations Made By | Dr. David Borchelt, University of Florida |
This strain originated on a B6;129S7 background and has been backcrossed to C57BL/6J for at least 5 generations (7/01).
When using the APLP2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004142 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Aplp2<tm1Dbo> |
Frozen Mouse Embryo | B6.129S7-Aplp2<tm1Dbo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Aplp2<tm1Dbo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Aplp2<tm1Dbo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Aplp2<tm1Dbo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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