Homozygous App mice weigh 15%-20% less than age-matched wild-type controls, and exhibit decreased locomotor activity and forelimb grip strength. Reactive gliosis is seen in some homozygous mice at 14 weeks of age. This beta-amyloid precursor protein (APP) knock-out strain offers a model useful in studies related to Alzheimer's disease.
Dr. David R Borchelt, University of Florida
Genetic Background | Generation |
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N20+N9F16
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | App | amyloid beta (A4) precursor protein |
At birth, mice homozygous for the targeted allele are viable, and do not display any gross physical or behavioral abnormalities. No App gene product (mRNA or protein) is detected. Body weight is 15-20% less than that observed in wildtype age-matched control mice. By 14 weeks of age the mice exhibit evidence of reactive gliosis. Neurological evaluation reveals significantly reduced forelimb grip strength and decreased locomotor activity. This mutant strain offers a model useful in studies related to Alzheimer's disease.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the App gene encoding the promoter and exon 1. The construct was electroporated into 129S7/SvEvBrd-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Allele Name | targeted mutation 1, David R Borchelt |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | App- |
Gene Symbol and Name | App, amyloid beta (A4) precursor protein |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 16 |
Molecular Note | A neomycin resistance cassette replaced a 3.8 kb sequence including the promoter region and first exon. Northern blot analysis did not detect mRNA in brain or kidney of homozygous mutant mice. |
Mutations Made By | Dr. David Borchelt, University of Florida |
This strain originated on a B6;129S7 background and has been backcrossed to C57BL/6J for at least ten generations (7/01). Coat color expected from breeding:Black
When using the B6.129S7-Apptm1Dbo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #004133 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or Wild-type for App<tm1Dbo> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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