Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM.
Robert Rickert, The Burnham Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Cd19 | CD19 antigen |
Marker Symbol | Marker Name | |
---|---|---|
Igh | immunoglobulin heavy chain complex |
The Cd19 promoter specifically directs expression at the earliest stages and throughout B-lymphocyte development and differentiation. A Cre cassette is inserted into the Cd19 exon 2, functionally disrupting the gene. Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Mice that are homozygous deficient for Cd19 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers.
Because the targeted mutation was generated on 129P2-derived embryonic stem cells, and the Cd19 locus is near the Tyr locus on chromosome 7, the these CD19-deficient mice may be albino (white coat color/pink eyes) or light-agouti coat color with dark eyes.
View cre expression characterization.
A targeting vector containing Cre recombinase, rabbit beta-globin intron /poly A signal sequence, and an FRT-flanked neomycin resistance gene, was used to disrupt exon 2 of the Cd19 gene. Herpes simplex virus thymidine kinase gene was placed 3' of the Cd19 sequence to allow for the selection against random integration. The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into 129/Sv blastocysts. This strain was backrossed to C.BKa-Ighb/IcrSmnJ (commonly known as C.B-17) for a minimum of 13 generations before being made homozygous.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | B cells |
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | B-Cre; CD19-; Cd19Cre; Cd19cre; CD19-Cre; CD19-KO |
Gene Symbol and Name | Cd19, CD19 antigen |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | B cells |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development. |
Mutations Made By | Robert Rickert, The Burnham Institute |
Marker Synonym(s) | |
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Chromosome(s) | 12 |
When using the CD19Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #004126 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd19<tm1(cre)Cgn> Igh<b> |
Frozen Mouse Embryo | C.Cg-Cd19<tm1(cre)Cgn> Igh<b>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.Cg-Cd19<tm1(cre)Cgn> Igh<b>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.Cg-Cd19<tm1(cre)Cgn> Igh<b>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.Cg-Cd19<tm1(cre)Cgn> Igh<b>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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