C.Cg-Cd19^tm1(cre)Cgn Igh^b/J

Stock number: 004126
Product name: CD19Cre
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM.

Detailed description

The Cd19 promoter specifically directs expression at the earliest stages and throughout B-lymphocyte development and differentiation. A Cre cassette is inserted into the Cd19 exon 2, functionally disrupting the gene. Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Mice that are homozygous deficient for Cd19 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers.

Because the targeted mutation was generated on 129P2-derived embryonic stem cells, and the Cd19 locus is near the Tyr locus on chromosome 7, the these CD19-deficient mice may be albino (white coat color/pink eyes) or light-agouti coat color with dark eyes.

View cre expression characterization.

Development

A targeting vector containing Cre recombinase, rabbit beta-globin intron /poly A signal sequence, and an FRT-flanked neomycin resistance gene, was used to disrupt exon 2 of the Cd19 gene. Herpes simplex virus thymidine kinase gene was placed 3' of the Cd19 sequence to allow for the selection against random integration. The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into 129/Sv blastocysts. This strain was backrossed to C.BKa-Igh^b/IcrSmnJ (commonly known as C.B-17) for a minimum of 13 generations before being made homozygous.

Allele

Symbol: Cd19^tm1(cre)Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1931143
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Cd19
Marker name: CD19 antigen
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: B cells
Molecular note: In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development.

Allele

MGI accession ID: MGI:96442
Marker symbol: Igh
Marker name: immunoglobulin heavy chain complex
Chromosome: 12