These mice carry an ENU-induced mutation characterized by subtle head-nodding and vestibular dysfunction.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) (Hypomorph) | Ankfn1 | ankyrin-repeat and fibronectin type III domain containing 1 |
The visible phenotype appears by weaning age (average age of onset 6 +/-1.1 weeks; n=18) as variable and generally subtle head-nodding. Mutants of either gender have been produced.
Nmf9 encodes a conserved protein that is functionally important to a distinct set of neurological phenotypes in both mice and Drosophila. Vestibular abnormalities are observed in addition to phenotypes in fear learning and circadian behavior. Nmf9 shows a complex pattern of expression in the vestibular system, olfactory system, and regions of the brain implicated in satiety and metabolism, innate anxiety, fear learning, and circadian rhythm. Females are more severely affected than males by the Nmf9 mutation. Mutations in the Nmf9 homologues of both mouse and fly result in phenotypes related to daily cycles of activity, as well as locomotor abnormalities. These mice may be useful in studies related to the evolutionary development of the nervous system.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
Allele Name | neuroscience mutagenesis facility, 9 |
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Allele Type | Chemically induced (ENU) (Hypomorph) |
Allele Synonym(s) | |
Gene Symbol and Name | Ankfn1, ankyrin-repeat and fibronectin type III domain containing 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 11 |
Molecular Note | ENU mutagenesis induced a G to A transition in the splice donor of a transcript that contains both Ankfn1 and 4932411E22Rik. RNA gel blot hybridization confirmed reduction in transcript expression in the brain. |
Homozygotes are viable and a colony could be maintained through homozygote or heterozygote matings.
When using the Nmf9 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004107 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Unknown for nmf9 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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