These mice carry an ENU-induced mutation characterized by subtle head-nodding and vestibular dysfunction.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The visible phenotype appears by weaning age (average age of onset 6 +/-1.1 weeks; n=18) as variable and generally subtle head-nodding. Mutants of either gender have been produced.
Nmf9 encodes a conserved protein that is functionally important to a distinct set of neurological phenotypes in both mice and Drosophila. Vestibular abnormalities are observed in addition to phenotypes in fear learning and circadian behavior. Nmf9 shows a complex pattern of expression in the vestibular system, olfactory system, and regions of the brain implicated in satiety and metabolism, innate anxiety, fear learning, and circadian rhythm. Females are more severely affected than males by the Nmf9 mutation. Mutations in the Nmf9 homologues of both mouse and fly result in phenotypes related to daily cycles of activity, as well as locomotor abnormalities. These mice may be useful in studies related to the evolutionary development of the nervous system.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 9|
|Allele Type||Chemically induced (ENU) (Hypomorph)|
|Gene Symbol and Name||Ankfn1, ankyrin-repeat and fibronectin type III domain containing 1|
|Strain of Origin||C57BL/6J|
|Molecular Note||ENU mutagenesis induced a G to A transition in the splice donor of a transcript that contains both Ankfn1 and 4932411E22Rik. RNA gel blot hybridization confirmed reduction in transcript expression in the brain.|
Homozygotes are viable and a colony could be maintained through homozygote or heterozygote matings.
When using the Nmf9 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004107 in your Materials and Methods section.