These F5 knock-out mice exhibit factor V deficiency resulting in neonatal massive intra-abdominal hemorrhage and death.
David Ginsburg, University of Michigan
Approximately half of the mice that are homozygous null for the F5 gene die at embryonic day 9-10. Those that develop beyond this stage continue to develop but suffer from massive intra-abdominal hemorrhages and die within two hours of birth. Also observed are microscopic hemorrhages in a variety of tissues. Blood present in the intra-abdominal cavity is unclotted and completely deficient of factor V activity.
A targeting vector containing a neomycin cassette designed to disrupt exons 8-11 was injected in 129S2/SvPas-derived D3 embryonic stems cells (ES). Correctly targeted ES were injected into C57BL/6J blastocysts and chimeric animals obtained. This strain originated on a B6;129 background and has been maintained as a heterozygote by backcrossing to C57BL/6J for at least 10 generations.
|Allele Name||targeted mutation 1, David Ginsburg|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||F5, coagulation factor V|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Replacement of exons 8-11 with a neomycin cassette.|
|Mutations Made By|| |
Jisong Cui, University of Michigan
This strain originated on a B6;129 background and has been maintained as a heterozygote by backcrossing to C57BL/6J. Backcrossing has proceeded to N10 (5/01).
When using the Fv- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004078 in your Materials and Methods section.