B6;129-Gt(ROSA)26Sor^tm2Sho/J

Stock number: 004077
Product name: ROSA26-EGFP^f
Research areas: Research Tools

Description

These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. The expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.

Detailed description

These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.

Development

A targeting vector containing the mouse phosphoglycerate kinase promoter driving cytosine deaminase and puromycin genes, a STOP fragment, an EGFP cDNA and a Bgeo gene (not expressed), was inserted into the Gt(ROSA)26Sor locus. Located immediately 5' of the puromycin gene, the stop fragment, and the EGFP cDNA are loxP sites. A herpes simplex viral thymidine kinase cassette was used as a negative selection marker. The construct was electroporated into ROSA26 ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.

Allele

Symbol: Gt(ROSA)26Sor^tm2Sho
Name: targeted mutation 2, Stuart Orkin
MGI accession ID: MGI:2136519
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Reporter
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S4/SvJaeSor
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues
Molecular note: These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in Cre-expressing tissues.