These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. The expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.
Stuart H Orkin, Harvard University
Genetic Background | Generation |
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F?+43
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.
A targeting vector containing the mouse phosphoglycerate kinase promoter driving cytosine deaminase and puromycin genes, a STOP fragment, an EGFP cDNA and a Bgeo gene (not expressed), was inserted into the Gt(ROSA)26Sor locus. Located immediately 5' of the puromycin gene, the stop fragment, and the EGFP cDNA are loxP sites. A herpes simplex viral thymidine kinase cassette was used as a negative selection marker. The construct was electroporated into ROSA26 ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues |
Allele Name | targeted mutation 2, Stuart Orkin |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | R26floxGFP; R26-GFP; RosaC; ROSA26-EGFP; ROSA26-EGFTf; ROSA26-flox-STOP-flox-GFP; Rosa26LOXPEGFP; Rosa26R-eGFP; ROSA-GFP; ROSA-loxP-stop-loxP-GFP loxP-GFP |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in Cre-expressing tissues. |
Mutations Made By | Dr. Xiaohong Mao, Novartis |
This strain originated on a B6;129 background and can be maintained as a hemizygote or homozygote. Coat color expected from breeding: Black
When using the ROSA26-EGFPf mouse strain in a publication, please cite the originating article(s) and include JAX stock #004077 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm2Sho> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Sho>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Sho>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Sho>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Sho>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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