These Crebbp knock-out mice exhibit embryonic lethality and heterozygotes show growth retardation, craniofacial abnormalities and failure to thrive. They may be useful in applications related to signal transduction pathways and studies of Rubinstein-Taybi syndrome (RTS) in humans.
Andrew L. Kung, Columbia University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Crebbp | CREB binding protein |
Mice that are homozygous null for the Crebbp gene are embryonic lethal, dying at about embryonic day 10.5 exhibiting open neural tube defects. Heterozygous mice though viable and fertile, are subject to early growth retardation, craniofacial abnormalities and often fail to thrive. Heterozygous mice are also subject to age-dependent splenomegaly and irregular hematopoiesis. By three months of age, the abundance of all hematopoietic cell types is significantly diminished. Older mice (10-21 months) are more prone to develop hematologic malignancies than wildtype mice.
Allele Name | targeted mutation 1, David Livingston |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CBP- |
Gene Symbol and Name | Crebbp, CREB binding protein |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 16 |
Molecular Note | Replacement of two exons encoding the CH1 domain of the Crebbp gene with a neomycin cassette. |
Mutations Made By | Andrew Kung, Columbia University |
Heterozygous mutant females are often unable to sustain entire litters successfully, usually to the detriment of mutant pups. Heterozygous pups are smaller than wild type and often fail to thrive. To increase the likelihood of mutant pup survival when utilizing mutant females for breeding, the donating investigator suggests removing wild type littermates shortly after birth. Alternatively, these problems can be avoided by breeding male mutants to wildtype females.
When using the CBP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004069 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Crebbp<tm1Dli> |
Frozen Mouse Embryo | B6.129S6-Crebbp<tm1Dli>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Crebbp<tm1Dli>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Crebbp<tm1Dli>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Crebbp<tm1Dli>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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