B6.129S2-Alox12^tm1Fun/J

Stock number: 004042
Product name: P-12LO-
Research areas: Cardiovascular Research, Dermatology Research, Hematological Research, Research Tools

Description

These Alox12 knock-out mice exhibit a hyper-responsiveness to adenosine diphosphate (ADP) -induced platelet aggregation and may be is useful in studies of thromboembolism.

Detailed description

Mice that are homozygous null for the Alox12 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Alox12 protein product or enzyme activity is detected in platelets derived from homozygous null animals. Platelets exhibit a hyperresponsiveness to ADP-induced aggregation. Studies examining basal transepidermal water loss indicate that null animals exhibit greater water loss through the skin when compared to control animals.

Development

A targeting vector containing a neomycin cassette was used to disrupt exon 8. The construct was electroporated into 129S2/SvPas-derived D3H embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric animals were obtained.

Allele

Symbol: Alox12^tm1Fun
Name: targeted mutation 1, Colin D Funk
MGI accession ID: MGI:1932927
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Alox12
Marker name: arachidonate 12-lipoxygenase
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette replaced exon 8. Western blot analysis did not detect the encoded protein in homozygous mutant platelets.