Mice that are homozygous null for the Zp3 gene are viable, normal in size and do not display any gross physical or behavioral abnormalities. No Zp3 gene product (mRNA or protein) is detected. Homozygous females are not fertile; they lack a zona pellucida and exhibit a disorganized corona radiata. Heterozygous females are fertile. Homozygous males appear normal in all respects and are fertile.
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the Zp3 exon 1. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were backcrossed to C57BL/6N mice.
|Allele Name||targeted mutation 1, Jurrien Dean|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Zp3, zona pellucida glycoprotein 3|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin cassette was inserted into exon 1. In situ hybridization experiments demonstrated an absence of mRNA in oocytes and the protein was undetectable in immunofluorescence experiments on mutant ovaries and oocytes.|
|Mutations Made By|| |
Jurrien Dean, NIH / NIDDK
This strain originated on a B6;129 background and has been backcrossed to C57BL/6N for at least ten generations. It is maintained by breeding homozygote males to wildtype females. Coat color expected from breeding:Black
When using the Zp3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004038 in your Materials and Methods section.